SPATIAL EXPRESSION OF MATRIX AND MATRIX RECEPTOR PROTEINS IN THE DEVELOPING SYNOVIAL JOINT

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dc.contributor.advisorCapehart, Anthony A.en_US
dc.contributor.authorRobins, Joshua Elien_US
dc.contributor.departmentBiology: Molecular Biology and Biotechnologyen_US
dc.date.accessioned2013-08-24T18:26:57Z
dc.date.available2016-05-11T21:42:04Z
dc.date.issued2013en_US
dc.description.abstractLittle is understood about the complex spatial and temporal molecular interactions necessary to form a fully functional synovial joint. Understanding molecular pathways and signaling within the developing joint continues to be an area of constant discovery. Versican is a molecule with strong expression in the extracellular matrix (ECM) that has proven necessary for proper joint development. The current study set out to discover gene expression patterns of ECM genes affected by the manipulation of versican. Five genes were chosen based on fold change differences identified from the results of an in-house 2010 microarray study. The microarray study evaluated gene expression differences when the G1 domain of versican was up-regulated in developing joint tissues using a recombinant adenoviral vector, and when the gene was silenced using adeno-shRNA. The five genes of interest were thrombospondin-3, discoidin domain receptor-2, sushi domain containing 5, matrix remodeling associated 5 and matrilin-4. In situ hybridization was performed on sagittal wing sections of HH35 (e9) chick embryos in an attempt to localize gene expression within the developing elbow joint. Results demonstrate gene expression was present in the developing elbow region for each of the five genes of interest. Furthermore mRNA localization for each of the five genes extended into surrounding tissues including the ectoderm, dermis, skeletal muscle, perichondrium, and chondrocytes. This study was undertaken to provide further insight into potential downstream effects of versican activity within the developing joint, as well as provide additional information on the presently limited knowledge surrounding the 5 genes of interest and their role in joint development.en_US
dc.description.degreeM.S.en_US
dc.format.extent95 p.en_US
dc.format.mediumdissertations, academicen_US
dc.identifier.urihttp://hdl.handle.net/10342/4194
dc.language.isoen_US
dc.publisherEast Carolina Universityen_US
dc.subjectBiologyen_US
dc.subjectDevelopmental biologyen_US
dc.subjectMolecular biologyen_US
dc.subjectBiology, Molecular
dc.subject.lcshGene expression
dc.subject.lcshGenetic regulation
dc.subject.lcshJoints
dc.subject.lcshEmbryology
dc.titleSPATIAL EXPRESSION OF MATRIX AND MATRIX RECEPTOR PROTEINS IN THE DEVELOPING SYNOVIAL JOINTen_US
dc.typeMaster's Thesisen_US

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