Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing
dc.contributor.author | Filippova, Elena M. | en_US |
dc.contributor.author | Monteleone, Denise C. | en_US |
dc.contributor.author | Trunk, John G. | en_US |
dc.contributor.author | Sutherland, Betsy M. | en_US |
dc.contributor.author | Quake, Stephen R. | en_US |
dc.contributor.author | Sutherland, John C. | en_US |
dc.date.accessioned | 2011-02-28T21:14:10Z | en_US |
dc.date.accessioned | 2011-05-17T15:07:50Z | |
dc.date.available | 2011-02-28T21:14:10Z | en_US |
dc.date.available | 2011-05-17T15:07:50Z | |
dc.date.issued | 2003-02 | en_US |
dc.description.abstract | Fluorescence from a single DNA molecule passing through a laser beam is proportional to the size (contour length) of the molecule, and molecules of different sizes can be counted with equal efficiencies. Single-molecule fluorescence can thus determine the average length of the molecules in a sample and hence the frequency of double-strand breaks induced by various treatments. Ionizing radiation-induced frank double-strand breaks can thus be quantified by single-molecule sizing. Moreover, multiple classes of clustered damages involving damaged bases and abasic sites, alone or in combination with frank single-strand breaks, can be quantified by converting them to double-strand breaks by chemical or enzymatic treatments. For a given size range of DNA molecules, single-molecule sizing is as or more sensitive than gel electrophoresis, and requires several orders-of-magnitude less DNA to determine damage levels. Originally published Biophysical Journal, Vol. 84, No. 2, Feb 2003 | en_US |
dc.identifier.citation | Biophysical Journal; 84:2 p. 1281-1290 | en_US |
dc.identifier.pmid | PMC1302704 | en_US |
dc.identifier.uri | http://hdl.handle.net/10342/3265 | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | East Carolina University | en_US |
dc.relation.uri | http://www.cell.com/biophysj/ | en_US |
dc.rights | Author notified of opt-out rights by Cammie Jennings. | en_US |
dc.subject | Laser fluorescence sizing | en_US |
dc.subject | DNA | en_US |
dc.subject | Double-strand breaks | en_US |
dc.subject | Cluster damage | en_US |
dc.title | Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing | en_US |
dc.type | Article | en_US |
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