Contractile activity restores insulin responsiveness in skeletal muscle of obese Zucker rats.

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dc.contributor.authorDolan, Patricia L.en_US
dc.contributor.authorTapscott, Edward B.en_US
dc.contributor.authorDorton, Peter J.en_US
dc.contributor.authorDohm, G. Lynisen_US
dc.date.accessioned2011-02-28T20:02:21Zen_US
dc.date.accessioned2011-05-17T00:56:26Z
dc.date.available2011-02-28T20:02:21Zen_US
dc.date.available2011-05-17T00:56:26Z
dc.date.issued1993-01-15en_US
dc.description.abstractBoth insulin and contraction stimulate glucose transport in skeletal muscle. Insulin-stimulated glucose transport is decreased in obese humans and rats. The aims of this study were (1) to determine if contraction-stimulated glucose transport was also compromised in skeletal muscle of genetically obese insulin-resistant Zucker rats, and (2) to determine whether the additive effects of insulin and contraction previously observed in muscle from lean subjects were evident in muscle from the obese animals. To measure glucose transport, hindlimbs from lean and obese Zucker rats were perfused under basal, insulin-stimulated (0.1,uM), contraction- stimulated (electrical stimulation of the sciatic nerve) and combined insulin-+contraction-stimulated conditions. One hindlimb was stimulated to contract while the contralateral leg served as an unstimulated control. 2-Deoxyglucose transport rates were measured in the white gastrocnemius, red gastrocnemius and extensor digitorum longus muscles. As expected, the insulin-stimulated glucose transport rate in each of the three muscles was significantly slower (P<0.05) in obese rats when compared with lean animals. When expressed as fold stimulation over basal, there was no significant difference in contraction-induced muscle glucose transport rates between lean-and obese animals. Insulin-+contraction-stimulation was additive in skeletal muscle of lean animals, but synergistic in skeletal muscle of obese animals. Prior contraction increased insulin responsiveness of glucose transport 2-5-fold in the obese rats, but had no effect on insulin responsiveness in the lean controls. This contraction-induced improvement in insulin responsiveness could be of clinical importance to obese subjects as a way to improve insulin-stimulated glucose uptake in resistant skeletal muscle. Originally published Biochemical Journal, Vol. 289, Pt. 2, Jan 1993en_US
dc.identifier.citationBiochemical Journal; 289:2 p. 423-426en_US
dc.identifier.doi10.1042/bj2890423
dc.identifier.pmidPMC1132184en_US
dc.identifier.urihttp://hdl.handle.net/10342/3262en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.biochemj.org/bj/default.htmen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings.en_US
dc.subjectGlucose transporten_US
dc.subjectObesityen_US
dc.subjectInsulin sensitivityen_US
dc.titleContractile activity restores insulin responsiveness in skeletal muscle of obese Zucker rats.en_US
dc.typeArticleen_US
ecu.journal.issue2
ecu.journal.nameBiochemical Journal
ecu.journal.pages423-426
ecu.journal.volume289

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