Identification of E2F1 as a positive transcriptional regulator for delta-catenin
dc.contributor.author | Kim, Kwonseop | en_US |
dc.contributor.author | Oh, Minsoo | en_US |
dc.contributor.author | Ki, Hyunkyoung | en_US |
dc.contributor.author | Wang, Tao | en_US |
dc.contributor.author | Bareiss, Sonja | en_US |
dc.contributor.author | Fini, M. Elizabeth | en_US |
dc.contributor.author | Li, Dawei | en_US |
dc.contributor.author | Lu, Qun | en_US |
dc.date.accessioned | 2011-04-15T15:11:31Z | en_US |
dc.date.accessioned | 2011-05-17T13:03:51Z | |
dc.date.available | 2011-04-15T15:11:31Z | en_US |
dc.date.available | 2011-05-17T13:03:51Z | |
dc.date.issued | 2008-05-02 | en_US |
dc.description.abstract | Delta-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate delta-catenin expression in cancer. Using a human delta-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect delta-catenin transcription. Among beta-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased delta-catenin-luciferase activities while beta-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of delta-catenin-luciferase activities induced by E2F1 but did not interact with delta-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of delta-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on delta-catenin expression were observed only in human cancer cells expressing abundant endogenous delta-catenin. These studies identify E2F1 as a positive transcriptional regulator for delta-catenin, but further suggest the presence of strong negative regulator(s) for delta-catenin in prostate cancer cells with minimal endogenous delta-catenin expression. Originally published Biochem Biophys REs Commun, Vol. 369, No. 2, May 2008 | en_US |
dc.identifier.citation | Biochemical and Biophysical Research Communications; 369:2 p. 414-420 | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2008.02.069 | |
dc.identifier.pmid | PMC2614404 | en_US |
dc.identifier.uri | http://hdl.handle.net/10342/3324 | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | East Carolina University | en_US |
dc.relation.uri | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4RXC89D-1&_user=634873&_coverDate=05%2F02%2F2008&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000033758&_version=1&_urlVersion=0&_userid=634873&md5=8b24218c2d1e2792f20a9e2998bf5a79&searchtype=a | en_US |
dc.rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
dc.subject | [Delta]-catenin/NPRAP | en_US |
dc.subject | E2F1 | en_US |
dc.subject | Wnt | en_US |
dc.subject | Notch | en_US |
dc.subject | Prostate | en_US |
dc.subject | Catenin | en_US |
dc.subject | LEF-1 | en_US |
dc.subject | Cancer | en_US |
dc.subject | Transcription | en_US |
dc.subject | δ-catenin/NPRAP | |
dc.title | Identification of E2F1 as a positive transcriptional regulator for delta-catenin | en_US |
dc.type | Article | en_US |
ecu.journal.issue | 2 | |
ecu.journal.name | 414-420 | |
ecu.journal.name | Biochemical and Biophysical Research Communications | |
ecu.journal.volume | 369 |
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