The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa

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dc.contributor.authorMcKnight, Susan L.en_US
dc.contributor.authorIglewski, Barbara H.en_US
dc.contributor.authorPesci, Everett C.en_US
dc.date.accessioned2011-04-15T19:10:37Zen_US
dc.date.accessioned2011-05-17T01:40:04Z
dc.date.available2011-04-15T19:10:37Zen_US
dc.date.available2011-05-17T01:40:04Z
dc.date.issued2000-05en_US
dc.description.abstractThe opportunistic pathogen Pseudomonas aeruginosa uses intercellular signals to control the density-dependent expression of many virulence factors. The las and rhl quorum-sensing systems function, respectively, through the autoinducers N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone (C4- HSL), which are known to positively regulate the transcription of the elastase-encoding gene, lasB. Recently, we reported that a second type of intercellular signal is involved in lasB induction. This signal was identified as 2-heptyl-3-hydroxy-4-quinolone and designated the Pseudomonas quinolone signal (PQS). PQS was determined to be part of the quorum-sensing hierarchy since its production and bioactivity depended on the las and rhl quorum-sensing systems, respectively. In order to define the role of PQS in the P. aeruginosa quorumsensing cascade, lacZ gene fusions were used to determine the effect of PQS on the transcription of the quorum-sensing system genes lasR, lasI, rhlR, and rhlI. We found that in P. aeruginosa, PQS caused a major induction of rhlI*-lacZ and had lesser effects on the transcription of lasR*-lacZ and rhlR*-lacZ. We also observed that the transcription of both rhlI*-lacZ and lasB*-lacZ was cooperatively effected by C4-HSL and PQS. Additionally, we present data indicating that PQS was not produced maximally until cultures reached the late stationary phase of growth. Taken together, our results imply that PQS acts as a link between the las and rhl quorum-sensing systems and that this signal is not involved in sensing cell density. Originally published Journal of Bacteriology, Vol. 182, No. 10, May 2000en_US
dc.identifier.citationJournal of Bacteriology; 182:10 p. 2702-2708en_US
dc.identifier.pmidPMC101972en_US
dc.identifier.urihttp://hdl.handle.net/10342/3355en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jb.asm.org/archive/2000.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectQuinolone signalen_US
dc.subjectLas quorum sensingen_US
dc.subjectRhl quorum sensingen_US
dc.titleThe Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosaen_US
dc.typeArticleen_US

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