Deep sequencing identifies novel and conserved microRNAs in peanuts (L.)

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dc.contributor.authorZhao, Chuan-Zhi
dc.contributor.authorXia, Han
dc.contributor.authorFrazier, Taylor Price
dc.contributor.authorYao, Ying-Yin
dc.contributor.authorBi, Yu-Ping
dc.contributor.authorLi, Ai-Qin
dc.contributor.authorLi, Meng-Jun
dc.contributor.authorLi, Chang-Sheng
dc.contributor.authorZhang, Bao-Hong
dc.contributor.authorWang, Xing-Jun
dc.date.accessioned2016-06-07T15:47:51Z
dc.date.available2016-06-07T15:47:51Z
dc.date.issued2010
dc.description.abstractBackground MicroRNAs (miRNAs) are a new class of small, endogenous RNAs that play a regulatory role in the cell by negatively affecting gene expression at the post-transcriptional level. miRNAs have been shown to control numerous genes involved in various biological and metabolic processes. There have been extensive studies on discovering miRNAs and analyzing their functions in model species, such as Arabidopsis and rice. Increasing investigations have been performed on important agricultural crops including soybean, conifers, and Phaselous vulgaris but no studies have been reported on discovering peanut miRNAs using a cloning strategy. Results In this study, we employed the next generation high through-put Solexa sequencing technology to clone and identify both conserved and species-specific miRNAs in peanuts. Next generation high through-put Solexa sequencing showed that peanuts have a complex small RNA population and the length of small RNAs varied, 24-nt being the predominant length for a majority of the small RNAs. Combining the deep sequencing and bioinformatics, we discovered 14 novel miRNA families as well as 75 conserved miRNAs in peanuts. All 14 novel peanut miRNAs are considered to be species-specific because no homologs have been found in other plant species except ahy-miRn1, which has a homolog in soybean. qRT-PCR analysis demonstrated that both conserved and peanut-specific miRNAs are expressed in peanuts. Conclusions This study led to the discovery of 14 novel and 22 conserved miRNA families from peanut. These results show that regulatory miRNAs exist in agronomically important peanuts and may play an important role in peanut growth, development, and response to environmental stress.en_US
dc.identifier.citationBMC Plant Biology; 10: p. 3-3en_US
dc.identifier.doi10.1186/1471-2229-10-3
dc.identifier.issn1471-2229
dc.identifier.pmidpmc2826338en_US
dc.identifier.urihttp://hdl.handle.net/10342/5478
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826338/en_US
dc.titleDeep sequencing identifies novel and conserved microRNAs in peanuts (L.)en_US
dc.typeArticleen_US
ecu.journal.nameBMC Plant Biologyen_US
ecu.journal.pages3-3en_US
ecu.journal.volume10en_US

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