Flow cytometer apparatus for three dimensional diffraction imaging and related methods
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Date
2009-12-17
Authors
Hu, Xin-hua
Jacobs, Kenneth
Lu, Jun
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Abstract
A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate a particle in the inner core fluid. A detector is configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source. An analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.