Opsonized Virulent Brucella abortus Replicates within Nonacidic, Endoplasmic Reticulum-Negative, LAMP-1-Positive Phagosomes in Human Monocytes

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dc.contributor.authorBellaire, Bryan H.en_US
dc.contributor.authorRoop, R. Martin IIen_US
dc.contributor.authorCardelli, James A.en_US
dc.date.accessioned2011-02-04T19:59:13Zen_US
dc.date.accessioned2011-05-17T01:40:01Z
dc.date.available2011-02-04T19:59:13Zen_US
dc.date.available2011-05-17T01:40:01Z
dc.date.issued2005-06en_US
dc.description.abstractCells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucellaspecific antibodies. To determine the effect of antibody opsonization on the ability of Brucella to establish itself within monocytes, the intracellular trafficking of virulent Brucella abortus 2308 and attenuated hfq and bacA mutants was followed in the human monocytic cell line THP-1. Early trafficking events of B. abortus 2308- containing phagosomes (BCP) were indistinguishable from those seen for control particles (heat-killed B. abortus 2308, live Escherichia coli HB101, or latex beads). All phagosomes transiently communicated the early-endosomal compartment and rapidly matured into LAMP-1 , cathepsin D , and acidic phagosomes. By 2 h postinfection, however, the number of cathepsin D BCP was significantly lower for live B. abortus 2308-infected cells than for either Brucella mutant strains or control particles. B. abortus 2308 persisted within these cathepsin D , LAMP-1 , and acidic vesicles; however, at the onset of intracellular replication, the numbers of acidic B. abortus 2308 BCP decreased while remaining cathepsin D and LAMP-1 . In contrast to B. abortus 2308, the isogenic hfq and bacA mutants remained in acidic, LAMP-1 phagosomes and failed to initiate intracellular replication. Notably, markers specific for the host endoplasmic reticulum were absent from the BCPs throughout the course of the infection. Thus, opsonized B. abortus in human monocytes survives within phagosomes that remain in the endosomal pathway and replication of virulent B. abortus 2308 within these vesicles corresponds with an increase in intraphagosomal pH. Originally published Infection and Immunity, Vol. 73, No. 6, June 2005en_US
dc.identifier.citationInfection and Immunity; 73:6 p. 3702-3713en_US
dc.identifier.doi10.1128/IAI.73.6.3702-3713.2005
dc.identifier.pmidPMC1111828en_US
dc.identifier.urihttp://hdl.handle.net/10342/3204en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://iai.asm.org/cgi/content/abstract/73/6/3702en_US
dc.rightsAuthor notified of opt-out rights by Cammie Jenningsen_US
dc.subjectMonocytesen_US
dc.subjectOpsonizationen_US
dc.subjectBrucella abortusen_US
dc.titleOpsonized Virulent Brucella abortus Replicates within Nonacidic, Endoplasmic Reticulum-Negative, LAMP-1-Positive Phagosomes in Human Monocytesen_US
dc.typeArticleen_US

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