Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa

Simple item page
dc.contributor.authorWade, Dana S.en_US
dc.contributor.authorCalfee, M. Worthen_US
dc.contributor.authorRocha, Edson R.en_US
dc.contributor.authorLing, Elizabeth A.en_US
dc.contributor.authorEngstrom, Elanaen_US
dc.contributor.authorColeman, James P.en_US
dc.contributor.authorPesci, Everett C.en_US
dc.date.accessioned2011-01-27T17:10:04Zen_US
dc.date.accessioned2011-05-17T01:39:59Z
dc.date.available2011-01-27T17:10:04Zen_US
dc.date.available2011-05-17T01:39:59Z
dc.date.issued2005-07en_US
dc.description.abstractPseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in cystic fibrosis patients and is a major source of nosocomial infections. This bacterium controls many virulence factors by using two quorum-sensing systems, las and rhl. The las system is composed of the LasR regulator protein and its cell-to-cell signal, N-(3-oxododecanoyl) homoserine lactone, and the rhl system is composed of RhlR and the signal N-butyryl homoserine lactone. A third intercellular signal, the Pseudomonas quinolone signal (PQS; 2-heptyl-3-hydroxy-4-quinolone), also regulates numerous virulence factors. PQS synthesis requires the expression of multiple operons, one of which is pqsABCDE. Previous experiments showed that the transcription of this operon, and therefore PQS production, is negatively regulated by the rhl quorum-sensing system and positively regulated by the las quorum-sensing system and PqsR (also known as MvfR), a LysR-type transcriptional regulator protein. With the use of DNA mobility shift assays and beta-galactosidase reporter fusions, we have studied the regulation of pqsR and its relationship to pqsA, lasR, and rhlR. We show that PqsR binds the promoter of pqsA and that this binding increases dramatically in the presence of PQS, implying that PQS acts as a coinducer for PqsR. We have also mapped the transcriptional start site for pqsR and found that the transcription of pqsR is positively regulated by lasR and negatively regulated by rhlR. These results suggest that a regulatory chain occurs where pqsR is under the control of LasR and RhlR and where PqsR in turn controls pqsABCDE, which is required for the production of PQS. Originally published Journal of Bacteriology, Vol. 187, No. 13, July 2005en_US
dc.identifier.citationJournal of Bacteriology; 187:13 p. 4372-4380en_US
dc.identifier.pmidPMC1151766en_US
dc.identifier.urihttp://hdl.handle.net/10342/3109en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jb.asm.org/archive/2005.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectQuinolone signal synthesisen_US
dc.subjectChronic lung infectionsen_US
dc.subjectCystic Fibrosisen_US
dc.titleRegulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosaen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Regulation fo pseudomonas quinolone.pdf
Size:
302.64 KB
Format:
Adobe Portable Document Format
Download

Collections

Microbiology and Immunology