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Identification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptor

dc.contributor.authorZhu, Yongen_US
dc.contributor.authorBond, Jason E.en_US
dc.contributor.authorThomas, Peteren_US
dc.date.accessioned2011-01-27T15:47:06Zen_US
dc.date.accessioned2011-05-17T14:35:03Z
dc.date.available2011-01-27T15:47:06Zen_US
dc.date.available2011-05-17T14:35:03Z
dc.date.issued2003-03-04en_US
dc.description.abstractRecently we discovered a previously uncharacterized gene with the characteristics of a membrane progestin receptor (mPR) in a fish model, spotted seatrout. Here, we report the identification, cloning, and characteristics of other members of this hitherto unknown family of putative mPRs from several vertebrate species, including human, mouse, pig, Xenopus, zebrafish, and Fugu, with highly conserved nucleotide and deduced amino acid sequences and similar structures to the spotted seatrout mPR. The 13 vertebrate genes identified seem to belong to an unknown gene family. Phylogenetic analysis indicates these cDNAs comprise three distinct groups (named alpha, beta, and gamma) within this gene family. Structural analyses of the translated cDNAs suggest they encode membrane proteins with seven transmembrane domains. The transcript sizes of the human alpha, beta, and gamma putative mPR mRNAs varied from 2.8 to 5.8 kb and showed distinct distributions in reproductive, neural, kidney and intestinal tissues, respectively. Recombinant human alpha, gamma, and mouse beta proteins produced in an Escherichia coli expression system demonstrated high affinity (Kd = 20 - 30 nM) saturable binding for progesterone. Further analysis of binding to the gamma - subtype revealed binding was specific for progestins and was displaceable, with rapid rates of association and dissociation (t1/2 = 2 - 8 min). These results suggest this is a new family of steroid receptors unrelated to nuclear steroid receptors, but instead having characteristics of G protein-coupled receptors. Originally published Proceedings of the National Academy of Sciences, Vol. 100, No. 5, 2237-2242en_US
dc.identifier.citationProceedings of the National Academy of Sciences; 100:5 p. 2237-2242en_US
dc.identifier.doi10.1073/pnas.0436133100
dc.identifier.pmidPMC151324en_US
dc.identifier.urihttp://hdl.handle.net/10342/3100en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.pnas.org/content/100/5/2237en_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectMembrane progestin receptoren_US
dc.subjectSteroid receptorsen_US
dc.subjectCDNAen_US
dc.titleIdentification, classification, and partial characterization of genes in humans and other vertebrates homologous to a fish membrane progestin receptoren_US
dc.typeArticleen_US
ecu.journal.issue5
ecu.journal.nameProceedings of the National Academy of Sciences
ecu.journal.pages2237-2242
ecu.journal.volume100

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