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Absence of unsaturated fatty acid synthesis in murine T lymphocytes.

dc.contributor.authorButtke, Thomas M.en_US
dc.contributor.authorVan Cleave, Stevenen_US
dc.contributor.authorSteelman, Lindaen_US
dc.contributor.authorMcCubrey, James A.en_US
dc.date.accessioned2011-02-17T15:46:20Zen_US
dc.date.accessioned2011-05-17T01:40:06Z
dc.date.available2011-02-17T15:46:20Zen_US
dc.date.available2011-05-17T01:40:06Z
dc.date.issued1989-08en_US
dc.description.abstractStearic acid is toxic for T lymphocytes in vitro but has little effect on B lymphocytes. To investigate the molecular basis for this difference, purified murine T and B lymphocytes were compared for their abilities to incorporate and metabolize stearic acid. Unstimulated T and B cells incorporated identical amounts of stearic acid into six different phospholipids and four neutral lipids. After mitogen stimulation, fatty acid uptake was increased in both lymphocyte types, but cell-specific differences were seen in the distribution of stearic acid among the various cellular lipids. Doses of stearic acid that selectively inhibited T-cell proliferation resulted in a 5-fold greater accumulation of distearoylphosphatidylcholine in T cells than in B cells. Whereas T cells did not desaturate the exogenously derived stearic acid, up to 25% of the saturated fatty acid was converted to oleic acid in B cells. These findings suggested a deficiency of stearoyl-CoA desaturase (acyl-CoA, hydrogen-donor:oxygen oxidoreductase, EC 1.14.99.5) activity in T cells, which was confirmed by subsequent studies. Cell-free extracts from B cells displayed nearly 20-fold more stearoyl- CoA desaturase activity than T-cell extracts, and the level of stearoyl-CoA desaturase mRNA was 30-fold higher in B cells. Collectively, our data indicate that murine T cells are deficient in unsaturated fatty acid synthesis. The deficiency of stearoyl- CoA desaturase in T cells may represent the basis for the differing sensitivities of T and B lymphocytes to inhibition by saturated fatty acids. Originally published Proceedings of the National Academy of Sciences, Vol. 86, No. 16, Aug 1989en_US
dc.identifier.citationProceedings of the National Academy of Sciences; 86:16 p. 6133-6137en_US
dc.identifier.pmidPMC297790en_US
dc.identifier.urihttp://hdl.handle.net/10342/3237en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://www.pnas.org/content/by/year/1989en_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectStearic aciden_US
dc.subjectLymphocytesen_US
dc.subjectSaturated fatty acidsen_US
dc.subjectUnsaturated fatty acidsen_US
dc.titleAbsence of unsaturated fatty acid synthesis in murine T lymphocytes.en_US
dc.typeArticleen_US
ecu.journal.issue16
ecu.journal.nameProceedings of the National Academy of Sciences
ecu.journal.pages6133-6137
ecu.journal.volume86

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