Absence of unsaturated fatty acid synthesis in murine T lymphocytes.
dc.contributor.author | Buttke, Thomas M. | en_US |
dc.contributor.author | Van Cleave, Steven | en_US |
dc.contributor.author | Steelman, Linda | en_US |
dc.contributor.author | McCubrey, James A. | en_US |
dc.date.accessioned | 2011-02-17T15:46:20Z | en_US |
dc.date.accessioned | 2011-05-17T01:40:06Z | |
dc.date.available | 2011-02-17T15:46:20Z | en_US |
dc.date.available | 2011-05-17T01:40:06Z | |
dc.date.issued | 1989-08 | en_US |
dc.description.abstract | Stearic acid is toxic for T lymphocytes in vitro but has little effect on B lymphocytes. To investigate the molecular basis for this difference, purified murine T and B lymphocytes were compared for their abilities to incorporate and metabolize stearic acid. Unstimulated T and B cells incorporated identical amounts of stearic acid into six different phospholipids and four neutral lipids. After mitogen stimulation, fatty acid uptake was increased in both lymphocyte types, but cell-specific differences were seen in the distribution of stearic acid among the various cellular lipids. Doses of stearic acid that selectively inhibited T-cell proliferation resulted in a 5-fold greater accumulation of distearoylphosphatidylcholine in T cells than in B cells. Whereas T cells did not desaturate the exogenously derived stearic acid, up to 25% of the saturated fatty acid was converted to oleic acid in B cells. These findings suggested a deficiency of stearoyl-CoA desaturase (acyl-CoA, hydrogen-donor:oxygen oxidoreductase, EC 1.14.99.5) activity in T cells, which was confirmed by subsequent studies. Cell-free extracts from B cells displayed nearly 20-fold more stearoyl- CoA desaturase activity than T-cell extracts, and the level of stearoyl-CoA desaturase mRNA was 30-fold higher in B cells. Collectively, our data indicate that murine T cells are deficient in unsaturated fatty acid synthesis. The deficiency of stearoyl- CoA desaturase in T cells may represent the basis for the differing sensitivities of T and B lymphocytes to inhibition by saturated fatty acids. Originally published Proceedings of the National Academy of Sciences, Vol. 86, No. 16, Aug 1989 | en_US |
dc.identifier.citation | Proceedings of the National Academy of Sciences; 86:16 p. 6133-6137 | en_US |
dc.identifier.pmid | PMC297790 | en_US |
dc.identifier.uri | http://hdl.handle.net/10342/3237 | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | East Carolina University | en_US |
dc.relation.uri | http://www.pnas.org/content/by/year/1989 | en_US |
dc.rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
dc.subject | Stearic acid | en_US |
dc.subject | Lymphocytes | en_US |
dc.subject | Saturated fatty acids | en_US |
dc.subject | Unsaturated fatty acids | en_US |
dc.title | Absence of unsaturated fatty acid synthesis in murine T lymphocytes. | en_US |
dc.type | Article | en_US |
ecu.journal.issue | 16 | |
ecu.journal.name | Proceedings of the National Academy of Sciences | |
ecu.journal.pages | 6133-6137 | |
ecu.journal.volume | 86 |
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