Lack of complex type N-glycans lessens aberrant neuronal properties
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Date
2018-06
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Authors
Hall, M. Kristen
Weidner, Douglas A.
Whitman, Austin A.
Schwalbe, Ruth A.
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Abstract
Modifications in surface glycans attached to proteins via N-acetylglucosamine-β1-Nasparagine linkage have been linked to tumor development and progression. These modifications include complex N-glycans with high levels of branching, fucose and sialic acid
residues. Previously, we silenced Mgat2 in neuroblastoma (NB) cells, which halted the
conversion of hybrid type N-glycans to complex type, to generate a novel cell line, NB_1
(-Mgat2). By comparing the aberrant cell properties of the NB_1(-Mgat2) cell line to
the parental cell line (NB_1), we investigated the impact of eliminating complex type N-glycans on NB cell behavior. Further, the N-glycosylation pathway in the NB_1(-Mgat2) cell
line was rescued by transiently transfecting cells with Mgat2, thus creating the NB_1
(-/+Mgat2) cell line. Changes in the N-glycosylation pathway were verified by enhanced
binding of E-PHA and L-PHA to proteins in the rescued cell line relative to those of the
NB_1(-Mgat2) cell line. Also, western blotting of total membranes from the rescued cell
line ectopically expressing a voltage-gated K+ channel (Kv3.1b) revealed that N-glycans
of Kv3.1b were processed to complex type. By employment of various cell lines, we demonstrated that reduction of the complex type N-glycans diminished anchorage-independent cell growth, and enhanced cell-cell interactions. Two independent cell invasion
assays showed that cell invasiveness was markedly lessened by lowering the levels of
complex type N-glycans while cell mobility was only slightly modified. Neurites of NB cells
were shortened by the absence of complex type N-glycans. Cell proliferation was reduced
in NB cells with lowered levels of complex type N-glycans which resulted from hindered
progression through G1+Go phases of the cell cycle. Overall, our results illustrate that
reducing the ratio of complex to hybrid types of N-glycans diminishes aberrant NB cell
behavior and thereby has a suppressive effect in cell proliferation, and cell dissociation
and invasion phases of NB.
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Hall, M. K., Weidner, D. A., Whitman, A. A., & Schwalbe, R. A. (2018). Lack of complex type N-glycans lessens aberrant neuronal properties. PloS One, 13(6), e0199202. doi:10.1371/journal.pone.0199202
DOI
10.1371/journal.pone.0199202 J