Repository logo
 

Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice

dc.contributor.authorShibata, Yoshimien_US
dc.contributor.authorFoster, L. Annen_US
dc.contributor.authorMetzger, Jamesen_US
dc.contributor.authorMyrvik, Quentin N.en_US
dc.date.accessioned2011-04-28T17:59:52Zen_US
dc.date.accessioned2011-05-17T00:56:28Z
dc.date.available2011-04-28T17:59:52Zen_US
dc.date.available2011-05-17T00:56:28Z
dc.date.issued1997-03en_US
dc.description.abstractIntravenous (i.v.) administration of phagocytosable chitin particles (1 to 10 mm) in C57BL/6 mice and SCID mice primed alveolar macrophages (Mf) within 3 days to yield up to a 50-fold increase in their oxidative burst when elicited in vitro with phorbol myristate acetate (PMA). C57BL/6 mice pretreated with monoclonal antibodies (MAbs) against mouse gamma interferon (IFN-g) or NK1.1 showed a markedly decreased level of alveolar Mf priming following injection of chitin particles. To confirm IFN-g production in vitro, spleen cells isolated from normal C57BL/6 mice and SCID mice were cultured with chitin particles. Significant IFN-g production was observed following stimulation with chitin but not with chitosan or latex beads. When spleen cells were treated with anti-NK1.1 MAb, IFN-g production was significantly inhibited. Another set of experiments showed that when C57BL/6 mice were pretreated i.v. with a small dose IFN-g, a higher level of priming was induced with not only phagocytosable chitin particles but also phagocytosable chitosan and even latex beads. Likewise, the spleen cell cultures preconditioned with IFN-g provided an up-regulation of IFN-g production by these phagocytosable particles. Taken together, the in vivo and in vitro results suggest that (i) the alveolar Mf priming mechanism is due, at least in part, to direct activation of Mf by IFN-g, which is produced by NK1.11 CD42 cells; (ii) IFN-g would have an autocrine-like effect on Mf and make them more responsive to particle priming; and (iii) phagocytosis of particulates, probably by a postmembrane event such as interiorization, appears to be important for the up-regulation of alveolar Mf priming and IFN-g production. Originally published Infection and Immunity, Vol. 65, No. 5, May 1997en_US
dc.identifier.citationInfection and Immunity; 65:5 p. 1734-1741en_US
dc.identifier.pmidPMC175208en_US
dc.identifier.urihttp://hdl.handle.net/10342/3404en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://iai.asm.org/archive/1997.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings prior to upload of this article.en_US
dc.subjectAlveolar macrophagesen_US
dc.subjectChitin particlesen_US
dc.subjectN-acetyl-D-glucosamineen_US
dc.titleAlveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in miceen_US
dc.typeArticleen_US
ecu.journal.issue5
ecu.journal.nameInfection and Immunity
ecu.journal.pages1734-1741
ecu.journal.volume65

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Alveolar macrophage priming intravenous.pdf
Size:
175.26 KB
Format:
Adobe Portable Document Format

Collections