The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa
dc.contributor.author | O'Toole, George A. | en_US |
dc.contributor.author | Gibbs, Karine A. | en_US |
dc.contributor.author | Hager, Paul W. | en_US |
dc.contributor.author | Phibbs, Paul V. Jr. | en_US |
dc.contributor.author | Kolter, Roberto | en_US |
dc.date.accessioned | 2011-04-28T14:30:03Z | en_US |
dc.date.accessioned | 2011-05-17T01:40:10Z | |
dc.date.available | 2011-04-28T14:30:03Z | en_US |
dc.date.available | 2011-05-17T01:40:10Z | |
dc.date.issued | 2000-01 | en_US |
dc.description.abstract | The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite repression control (Crc) protein, which plays a role in the regulation of carbon metabolism, is necessary for biofilm formation in Pseudomonas aeruginosa. Using phase-contrast microscopy, we found that a crc mutant only makes a dispersed monolayer of cells on a plastic surface but does not develop the dense monolayer punctuated by microcolonies typical of the wild-type strain. This is a phenotype identical to that observed in mutants defective in type IV pilus biogenesis. Consistent with this observation, crc mutants are defective in type IV pilus-mediated twitching motility. We show that this defect in type IV pilus function is due (at least in part) to a decrease in pilA (pilin) transcription. We propose that nutritional cues are integrated by Crc as part of a signal transduction pathway that regulates biofilm development. Originally published Journal of Bacteriology, Vol. 182, No. 2, Jan 2000 | en_US |
dc.identifier.citation | Journal of Bacteriology; 182:2 p. 425-431 | en_US |
dc.identifier.doi | 10.1128/JB.182.2.425-431.2000 | |
dc.identifier.pmid | PMC94292 | en_US |
dc.identifier.uri | http://hdl.handle.net/10342/3373 | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | East Carolina University | en_US |
dc.relation.uri | http://jb.asm.org/archive/2000.dtl | en_US |
dc.rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
dc.subject | Biofilms | en_US |
dc.subject | Pseudomonas aeruginosa | en_US |
dc.subject | Catabolite repression control | en_US |
dc.title | The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa | en_US |
dc.type | Article | en_US |
ecu.journal.issue | 2 | |
ecu.journal.name | Journal of Bacteriology | |
ecu.journal.pages | 425-431 | |
ecu.journal.volume | 182 |
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