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Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model

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Date

2004-08

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Authors

Alcantara, Rosemarie B.
Read, Richard D. A.
Valderas, Michelle Wright
Brown, Timothy D.
Roop, R. Martin II

Journal Title

Journal ISSN

Volume Title

Publisher

East Carolina University

Abstract

Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment. Originally published Infection and Immunity, Vol. 72, No. 8, Aug 2004

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Citation

Infection and Immunity; 72:8 p. 4911-4917

DOI

10.1128/IAI.72.8.4911-4917.2004