Spleen Tyrosine Kinase (Syk) Regulates Systemic Lupus Erythematosus (SLE) T Cell Signaling
| dc.contributor.author | Grammatikos, Alexandros P. | |
| dc.contributor.author | Ghosh, Debjani | |
| dc.contributor.author | Devlin, Amy | |
| dc.contributor.author | Kyttaris, Vasileios C. | |
| dc.contributor.author | Tsokos, George C. | |
| dc.date.accessioned | 2016-06-14T13:54:21Z | |
| dc.date.available | 2016-06-14T13:54:21Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Engagement of the CD3/T cell receptor complex in systemic lupus erythematosus (SLE) T cells involves Syk rather than the zeta-associated protein. Because Syk is being considered as a therapeutic target we asked whether Syk is central to the multiple aberrantly modulated molecules in SLE T cells. Using a gene expression array, we demonstrate that forced expression of Syk in normal T cells reproduces most of the aberrantly expressed molecules whereas silencing of Syk in SLE T cells normalizes the expression of most abnormally expressed molecules. Protein along with gene expression modulation for select molecules was confirmed. Specifically, levels of cytokine IL-21, cell surface receptor CD44, and intracellular molecules PP2A and OAS2 increased following Syk overexpression in normal T cells and decreased after Syk silencing in SLE T cells. Our results demonstrate that levels of Syk affect the expression of a number of enzymes, cytokines and receptors that play a key role in the development of disease pathogenesis in SLE and provide support for therapeutic targeting in SLE patients. | en_US |
| dc.identifier.citation | PLoS ONE; 8:8 p. 1-9 | en_US |
| dc.identifier.doi | 10.1371/journal.pone.0074550 | |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.pmid | pmc3754955 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10342/5577 | |
| dc.relation.uri | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074550 | en_US |
| dc.subject | T cells | en_US |
| dc.subject | Blood donors | en_US |
| dc.subject | Flow cytometry | en_US |
| dc.subject | Cytokines | en_US |
| dc.subject | Cytokines | en_US |
| dc.title | Spleen Tyrosine Kinase (Syk) Regulates Systemic Lupus Erythematosus (SLE) T Cell Signaling | en_US |
| dc.type | Article | en_US |
| ecu.journal.issue | 8 | en_US |
| ecu.journal.name | PLoS ONE | en_US |
| ecu.journal.pages | 1-9 | en_US |
| ecu.journal.volume | 8 | en_US |
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