Crc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa

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dc.contributor.authorHester, Kathryn L.en_US
dc.contributor.authorLehman, Jodien_US
dc.contributor.authorNajar, Faresen_US
dc.contributor.authorSong, Linen_US
dc.contributor.authorRoe, Bruce A.en_US
dc.contributor.authorMacGregor, Carolyn H.en_US
dc.contributor.authorHager, Paul W.en_US
dc.contributor.authorPhibbs, Paul V. Jren_US
dc.contributor.authorSokatch, John R.en_US
dc.date.accessioned2011-03-02T20:03:37Zen_US
dc.date.accessioned2011-05-17T01:40:03Z
dc.date.available2011-03-02T20:03:37Zen_US
dc.date.available2011-05-17T01:40:03Z
dc.date.issued2000-02en_US
dc.description.abstractCrc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of the bkd operon, were isolated and identified as crc and vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels. Originally published Journal of Bacteriology, Vol. 182, No. 4, Feb 2000en_US
dc.identifier.citationJournal of Bacteriology; 182:4 p. 1144-1149en_US
dc.identifier.doi10.1128/JB.182.4.1144-1149.2000
dc.identifier.pmidPMC94392en_US
dc.identifier.urihttp://hdl.handle.net/10342/3299en_US
dc.language.isoen_USen_US
dc.publisherEast Carolina Universityen_US
dc.relation.urihttp://jb.asm.org/archive/2000.dtlen_US
dc.rightsAuthor notified of opt-out rights by Cammie Jennings.en_US
dc.subjectCatabolite repression controlen_US
dc.subjectCarbon regulationen_US
dc.subjectPseudomonadsen_US
dc.titleCrc Is Involved in Catabolite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosaen_US
dc.typeArticleen_US
ecu.journal.issue4
ecu.journal.nameJournal of Bacteriology
ecu.journal.pages1144-1149
ecu.journal.volume182

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