Browsing by Author "Biology: Molecular Biology and Biotechnology"
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Analysis of Complex DNA Damage Associated with Tumor Growth
Ferguson, Nicholas Foster (East Carolina University, 2012)The innate response of a fully functioning immune system's ability to recognize and react to the growth of a tumor by releasing reactive oxygen species (ROS) may lead to chronic inflammation in many cases. This chronic ... -
Analysis of Myopodin in Hela Cervical Carcinoma Cells
Harkins, Melissa (East Carolina University, 2014)Synaptopodin-2 is a proline-rich, actin-binding protein that exists in many isoforms. Due to its large proline content, synaptopodin-2 exists in a naturally unfolded state and possesses the characteristics of a hub protein. ... -
Analysis of scn5Laa and scn5Lab Gene Function in Danio rerio (Zebrafish) Heart Development through TALENs/CRISPR-CAS9-mediated Gene Knockout
Chokshi, Payal (East Carolina University, 2015)Research in our laboratory is focused on understanding the molecular developmental genetic effects of crude oil exposure on vertebrate embryogenesis. Our research has demonstrated that exposure of zebrafish embryos to ... -
Analysis of the hex regulon in Pseudomonas aeruginosa
Williams, Melinda (East Carolina University, 2012)Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are required for catabolism of glucose ... -
Assessing cellular and genomic damage from environmental nickel using a GFP containing strain of Caenorhabditis elegans
Huffnagle, Ian M. (East Carolina University, 2013)Nickel is a naturally found mineral that has become widely used for many electronic devices. As use and subsequent discarding of nickel containing products continues exposure to nickel increases. Nickel can cause external ... -
CHARACTERIZATION OF MIGRATING NEURAL CREST CELLS FROM HINDBRAIN EXPLANTS IN ZEBRAFISH
Murolo, Daniel (East Carolina University, 2010)Our lab was interested in creating a reproducible mechanism for obtaining neural crest cells in culture from cultured hindbrain explants of zebrafish. We have characterized the population of cells migrating from the ... -
Characterization of MiR319-Regulated TCPs in Maize Development
Novitzky, Katherine (East Carolina University, 1/13/16)Maize produces two inflorescences, the tassel and the ear, that are essential for reproduction. Both inflorescences arise from similar inflorescence primordia and are patterned largely by the same developmental regulators. ... -
Characterization of Site-Directed Mutants in the Cytochrome c-550 Protein of Photosystem II
Manne, Akarsh (East Carolina University, 2010)Photosynthesis is the process by which cyanobacteria, algae, and higher plants convert light energy to chemical energy via the biosynthesis of carbohydrates. Photosystem II is a multi-protein/pigment complex embedded in ... -
Characterization of the GINS subunit psf1 in Drosophila Melanogaster
Sufrinko, Brian (East Carolina University, 2010)GINS (Go Ichi Ni San; 5,1,2,3 in Japanese) is a heterotetrameric protein complex known to be essential for the establishment of DNA replication forks and the progression of the replisome. The GINS complex is known to be ... -
Characterizing the Expression Pattern of miR167-Regulated ZmArf3 and ZmArf30 in Maize Inflorescences
Johnson, Caitlin E. (East Carolina University, 2014)Plant growth and development depends on the activity of meristems, pools of stem cells that generate leaves and other organs. Maize (corn) produces two inflorescences, the tassel and ear, which are patterned by a series ... -
Characterizing the vegetative phenotype of fzt maize mutant
Basham, Christine Elizabeth (East Carolina University, 2012)microRNAs are short, non-coding RNA strands that regulate gene expression post-transcriptionally in all multicellular organisms. miRNAs begin as a hairpin in the nucleus. The primary miRNA (pri-miRNA) and preliminary ... -
Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development
Nagchowdhuri, Partha S. (East Carolina University, 2012)The different mechanisms involved in vertebrate synovial joint development are actively being uncovered. A variety of studies have thus far discovered the involvement of several large molecules in the processes of joint ... -
DERIVATION OF TOLEROGENIC THERAPEUTIC VACCINES FOR MOUSE MODELS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
Islam, S.M. Touhidul (East Carolina University, 2012)Multiple sclerosis (MS) is a chronic inflammatory disease that results in autoimmune demyelination of the human central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE) is a widely-studied animal ... -
Determining the Chemical Structure of Brucebactin: The Sole Complex Siderophore Utilized by the Pathogenic Bacterium Brucella Abortus
Atkinson, Xavier J. (East Carolina University, 1/13/16)Brucellosis is a zoonotic bacterial disease that affects many around the world. There are over half a million new cases each year and prevalence rates in some areas of the world exceed 10 new cases per 100,000 in the ... -
DISINTEGRIN-LIKE DOMAIN IN KSHV ENCODED gB IS A NECESSITY TO PROMOTE VIRUS LATENCY
Walker, Lia R. (East Carolina University, 2011)KSHV glycoprotein B (gB) is a lytic structural protein expressed on the envelope of mature virions and on the membrane of cells supporting lytic infection. Previous studies have identified that in addition to the role of ... -
Drosophila Ctf4 is essential for genome stability and normal cell cycle progression
Gosnell, Justin A. (East Carolina University, 2010)Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding ... -
Drosophila Psf2 Has a Role In Chromosome Condensation
Chmielewski, Jeffrey Patrick (East Carolina University, 2012)The condensation state of chromosomes is a critical parameter in multiple processes within the cell. Failures in the maintenance of appropriate condensation states may lead to genomic instability, mis-expression of genes, ... -
Effects of 5-fluorouracil Drug Treatment on the Expression Profile of MicroRNAs in MCF7 Breast Cancer Cells
Shah, Maitri Yogen (East Carolina University, 2010)Breast cancer is one of the leading causes of deaths in women worldwide. 5-flourouracil (5-FU) is a classic chemotherapeutic drug that has been widely used in the treatment of breast cancer patients. In this study, using ... -
The effects of sodium butyrate on Hox gene expression in a human colorectal adenocarcinoma cell line, HT29.
Conaty, Lucy D. (East Carolina University, 2013)Hox genes are a subgroup of the large family of homeobox containing genes, known to pattern anterior/posterior and proximal/distal axes during embryonic development. More recently Hox gene research has focused on the role ... -
Elucidating Gene Function with the use of Genome Wide Association Study (GWAS)
Ransdell, Garrett (East Carolina University, 2015)DNA replication and cell proliferation are intensely studied subjects. There have been countless experiments and hours put into better understanding how DNA is replicated and compacted, and how cells are able to proliferate ...