2017-2018 Open Access Publishing Fund

Permanent URI for this collection

http://hdl.handle.net/10342/7360

Browse

Now showing 1 - 20 of 21

Open Access
Large Left Ventricular Thrombus as a Cause of Recurrent Cardioembolic Stroke While on Dabigatran
(2018-10) Moey, Melissa Y. Y.; Tomdio, Anna N.; Achike, Olisaemeka; Kabirdas, Deepa
Open Access
Software for objective comparison of vocal acoustic features over weeks of audio recording: KLFromRecordingDays
(2017-10-09) Soderstrom, Ken; Alalawi, Ali Mohammed
KLFromRecordingDays allows measurement of Kullback–Leibler (KL) distances between 2D probability distributions of vocal acoustic features. Greater KL distance measures reflect increased phonological divergence across the vocalizations compared. The software has been used to compare *.wav file recordings made by Sound Analysis Recorder 2011 of songbird vocalizations pre- and post-drug and surgical manipulations. Recordings from individual animals in *.wav format are first organized into subdirectories by recording day and then segmented into individual syllables uttered and acoustic features of these syllables using Sound Analysis Pro 2011 (SAP). KLFromRecordingDays uses syllable acoustic feature data output by SAP to a MySQL table to generate and compare “template” (typically pre-treatment) and “target” (typically post-treatment) probability distributions. These distributions are a series of virtual 2D plots of the duration of each syllable (as x -axis) to each of 13 other acoustic features measured by SAP for that syllable (as y -axes). Differences between “template” and “target” probability distributions for each acoustic feature are determined by calculating KL distance, a measure of divergence of the target 2D distribution pattern from that of the template. KL distances and the mean KL distance across all acoustic features are calculated for each recording day and output to an Excel spreadsheet. Resulting data for individual subjects may then be pooled across treatment groups and graphically summarized and used for statistical comparisons. Because SAP-generated MySQL files are accessed directly, data limits associated with spreadsheet output are avoided, and the totality of vocal output over weeks may be objectively analyzed all at once. The software has been useful for measuring drug effects on songbird vocalizations and assessing recovery from damage to regions of vocal motor cortex. It may be useful in studies employing other species, and as part of speech therapies tracking progress in producing distinct speech sounds in isolation.
Open Access
The Impact of Online Video Cases on Clinical Reasoning in Occupational Therapy Education: A Quantitative Analysis
(2018-07-01) Murphy, Lynne F.; Stav, Wendy B.
Clinical reasoning, the cognitive process of a skilled occupational therapist, is a complex and necessary component of evaluating clients and implementing interventions that facilitate each client’s achievement of relevant and meaningful participation in daily occupations. Clinical reasoning encompasses a set of skills that must be integrated into college curricula for the preparation of occupational therapists, but it is not easily taught in a classroom setting. This quasi-experimental, quantitative research explored how specific instructional techniques, constructed on the tenets of case-based reasoning, influenced the development of clinical reasoning in occupational therapy students. The experimental group with video cases improved significantly in overall reasoning. The video cases challenged the learners to analyze their observations, explicitly identify clinical reasoning skills, and make decisions regarding occupational therapy interventions and services. This may influence how video technology is used and integrated into didactic educational practices.
Open Access
Anatomical-Molecular Distribution of EphrinA1 in Infarcted Mouse Heart Using MALDI Mass Spectrometry Imaging
(2018-01-05) Lefcoski, Stephan; Kew, Kimberly; Reece, Shaun; Torres, Maria J.; Parks, Justin C.; de Castro Brás, Lisandra E.; Virag, Jitka A. I.
EphrinA1 is a tyrosine kinase receptor localized in the cellular membrane of healthy cardiomyocytes, the expression of which is lost upon myocardial infarction (MI). Intra-cardiac injection of the recombinant form of ephrinA1 (ephrinA1-Fc) at the time of ligation in mice has shown beneficial effects by reducing infarct size and myocardial necrosis post-MI. To date, immunohistochemistry and Western blotting comprise the only experimental approaches utilized to localize and quantify relative changes of ephrinA1 in sections and homogenates of whole left ventricle, respectively. Herein, we used matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) coupled with a time-of-flight mass spectrometer (MALDI/TOF MS) to identify intact as well as tryptic fragments of ephrinA1 in healthy controls and acutely infarcted murine hearts. The purpose of the present study was 3-fold: (1) to spatially resolve the molecular distribution of endogenous ephrinA1, (2) to determine the anatomical expression profile of endogenous ephrinA1 after acute MI, and (3) to identify molecular targets of ephrinA1-Fc action post-MI. The tryptic fragments detected were identified as the ephrinA1-isoform with 38% and 34% sequence coverage and Mascot scores of 25 for the control and MI hearts, respectively. By using MALDI-MSI, we have been able to simultaneously measure the distribution and spatial localization of ephrinA1, as well as additional cardiac proteins, thus offering valuable information for the elucidation of molecular partners, mediators, and targets of ephrinA1 action in cardiac muscle.
Open Access
Factors Affecting Patient Portal Use Among Low-Income Pregnant Women: Mixed-Methods Pilot Study
(2018-03) Kim, Juhee; Mathews, Holly F.; Cortright, Lindsay M.; Zeng, Xiaoming; Newton, Edward
Background: Patient portals offer patients personalized and secure Web access to their medical information and enable patients to manage their health care online. However, there is a lack of information about patient acceptance and use of patient portals among low-income pregnant women. Objective: This formative research aims to assess the potential of a patient portal, MyChart, for improving prenatal health care and pregnancy outcomes, and identify the barriers and facilitators of MyChart use among low-income pregnant women. Methods: A mixed-methods study was conducted with a convenience sample of 18 low-income pregnant women comprising low- and high-risk patients enrolled in a prenatal clinic in eastern North Carolina. MyChart use, patient demographics, and pregnancy information were collected by reviewing electronic medical charts. Health literacy was measured. Reported use and attitudes toward MyChart were collected using a semi-structured interview. Results: Although 39% (7/18) of participants interviewed signed up for MyChart, only 22% (4/18) of them became active users. Another 33% (6/18) had never heard of MyChart or was unsure of how to access it. Users primarily accessed test results and appointment schedules. The main facilitating factors for patient portal use were information and motivation from health care providers and concerns about pregnancy due to a history of miscarriage. Reported barriers were lack of educational resources, lack of care provider encouragement, and technical difficulties possibly exacerbated by low health literacy. Participants also suggested improvements for MyChart, especially the provision of discussion-based support for pregnant women. Conclusions: The one-time verbal introduction of MyChart does not meet current patients’ needs. Data reveal the need for more consistent patient education and support programs, tailored to patients’ previous pregnancy histories. The clinic also needs to facilitate better provider-patient communication about the importance of MyChart use.
Open Access
Recommendations for active correction of hypernatremia in volume-resuscitated shock or sepsis patients should be taken with a grain of salt: A systematic review
(2018-03-21) Quinn, Joseph W.; Sewell, Kerry; Simmons, Dell E.
Background: Healthcare-acquired hypernatremia (serum sodium >145 mEq/dL) is common among critically ill and other hospitalized patients and is usually treated with hypotonic fluid and/or diuretics to correct a “free water deficit.” However, many hypernatremic patients are eu- or hypervolemic, and an evolving body of literature emphasizes the importance of rapidly returning critically ill patients to a neutral fluid balance after resuscitation. Objective: We searched for any randomized- or observational-controlled studies evaluating the impact of active interventions intended to correct hypernatremia to eunatremia on any outcome in volume-resuscitated patients with shock and/or sepsis. Data sources: We performed a systematic literature search with studies identified by searching MEDLINE, Embase, Cochrane Central Register of Controlled Trials, Cochrane Database of Systematic Reviews, ClinicalTrials.gov, IndexCatalogue of the Library of the Surgeon General’s Office, DARE (Database of Reviews of Effects), and CINAHL and scanning reference lists of relevant articles with abstracts published in English. Data synthesis: We found no randomized- or observational-controlled trials measuring the impact of active correction of hypernatremia on any outcome in resuscitated patients. Conclusion: Recommendations for active correction of hypernatremia in resuscitated patients with sepsis or shock are unsupported by clinical research acceptable by modern evidence standards.
Open Access
protaTETHER – a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc‐GFP fusion protein
(2018-03-14) Norris, Jessica L.; Hughes, Robert M.
Protein fusions are of fundamental importance in the study of cellular biology and the elucidation of cell signaling pathways, and the importance of linkers for the proper function of protein fusions is well documented in the literature. However, there are few convenient methods available to experimentalists for the systematic implementation of linkers in protein fusions. In this work, we describe a universal approach to the creation and insertion of focused linker libraries into protein fusions. This process, deemedprotaTETHER, utilizes reiterative oligomer design, PCR-mediated linker library generation, and restriction enzyme-free cloning methods in a straightforward, three-step cloning process. We utilize a fusion between the catalytic subunit of cAMP-dependent protein kinase A (PKAc) and green fluorescent protein (GFP) for the development of the protaTETHERmethod, implementing small linker libraries that vary by length, sequence, and predicted secondary structural elements. We analyze the impact of linker length and sequence on the expression, activity, and subcellular localization of the PKAc-GFP fusions, and use these results to select a PKAc-GFP fusion construct with robust expression and enzymatic activity. Based upon the results of both biochemical experiments and molecular modeling, we determine that linker flexibility is more important than linker length for optimal kinase activity and expression.
Open Access
Wetland management strategies lead to tradeoffs in ecological structure and function
(2017-12) Peralta, Ariane L.; Muscarella, Mario E.; Matthews, Jeffrey W.
Anthropogenic legacy effects often occur as a consequence of land use change or land management and can leave behind long-lasting changes to ecosystem structure and function. This legacy is described as a memory in the form of ecological structure or ecological interactions that remains at a location from a previous condition. We examined how forested floodplain restoration strategy, based on planting intensity, influenced wetland community structure and soil chemical and physical factors after 15 years. The site was divided into 15 strips, and strips were assigned to one of five restoration treatments: plantings of acorns, 2-year-old seedlings, 5-ft bareroot trees, balled and burlapped trees, and natural seed bank regeneration. Our community composition survey revealed that plots planted with bareroot or balled and burlapped trees developed closed tree canopies with little herbaceous understory, while acorn plantings and natural colonization plots developed into dense stands of the invasive species reed canary grass (RCG; Phalaris arundinacea). Restoration strategy influenced bacterial community composition but to a lesser degree compared to the plant community response, and riverine hydrology and restoration strategy influenced wetland soil conditions. Soil ammonium concentrations and pH were similar across all wetland restoration treatments, while total organic carbon was highest in forest and RCG-dominated plots compared to mixed patches of trees and open areas. The differences in restoration strategy and associated economic investment resulted in ecological tradeoffs. The upfront investment in larger, more mature trees (i.e., bareroot, balled and burlapped) led to floodplain forested communities, while cheaper, more passive planting strategies (i.e., seedlings, seedbank, or acorns) resulted in dense stands of invasive RCG, despite the similar floodplain hydrology across all sites. Therefore, recovery of multiple ecosystem services that encompass plant and microbial-derived functions will need to include additional strategies for the recovery of plants, microbes, environment, and functions.
Open Access
Impaired T cell-mediated hepatitis in peroxisome proliferator activated receptor alpha (PPARα)-deficient mice
(2018-02-15) Hines, Ian N.; Kremer, Michael; Moore, Sherri M.; Wheeler, Michael D.
Background Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). Methods Wild type (wt) or PPARα-deficient (PPARα−/−) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization. Results Ten and 24 h following ConA administration, wt mice had significant liver injury as demonstrated by serum transaminase levels, inflammatory cell infiltrate, hepatocyte apoptosis, and expression of several cytokines including interleukin 4 (IL4) and interferon gamma (IFNγ). In contrast, PPARα−/− mice were protected from ConA-induced liver injury with significant reductions in serum enzyme release, greatly reduced inflammatory cell infiltrate, hepatocellular apoptosis, and IFNγ expression, despite having similar levels of hepatic T cell activation and IL4 expression. This resistance to liver injury was correlated with reduced numbers of hepatic natural killer T (NKT) cells and their in vivo responsiveness to alpha-galactosylceramide. Interestingly, adoptive transfer of either wt or PPARα−/− splenocytes reconstituted ConA liver injury and cytokine production in lymphocyte-deficient, severe combined immunodeficient mice implicating PPARα within the liver, possibly through support of IL15 expression and/or suppression of IL12 production and not the lymphocyte as the key regulator of T cell activity and ConA-induced liver injury. Conclusion Taken together, these data suggest that PPARα within the liver plays an important role in ConA-mediated liver injury through regulation of NKT cell recruitment and/or survival.
Open Access
An Experiment Assessing Punitive versus Wellness Framing of a Tobacco-Free Campus Policy on Students’ Perceived Level of University Support
(2017-08-20) Lee, Joseph G. L.; Purcell, Christopher J.; Chaney, Beth H.
The objective of this study was to examine how different ways of describing a hypothetical tobacco-free campus policy would impact college students’ perceived level of support from the college. In the spring of 2016, we randomized 1885 undergraduate students in a required course to three message conditions in an online survey: control (no message), wellness (emphasizing promoting health and quitting support), and punitive (emphasizing consequences for violating the policy). The dependent variable was perceived organizational support. We selected items previously shown to be relevant for college students (alpha = 0.92 in our data). Given significant non-normality, we used non-parametric Kruskal-Wallis tests with pairwise comparisons to examine differences in perceived organizational support across the three conditions. We examined results by smoking status and if the participant correctly reported the message they received. We found no significant difference in perceived organizational support among students exposed to different tobacco-free campus policy announcements (p = 0.75). We also found no significant difference among smokers (p = 0.66). However, among smokers who correctly reported the message they received, we found significantly lower perceived university support (p = 0.01). Messages about tobacco-free campus policies should focus on the role of policy in supporting a healthy environment instead of punitive enforcement. Campus administrators should use caution when using message frames focusing on consequences of violating newly adopted policies.
Open Access
A second locality for the Namib darkling beetle Onymacris brainei (Tenebrionidae, Coleoptera) and first report on its molecular phylogenetic placement
(2017-08-01) Lamb, Trip; Marais, Eugene; Bond, Jason E.
Onymacris brainei Penrith, 1984 – the most recent species of Onymacris to be described – is known only from its type locality in the Namib Desert, adjacent to the Cunene River in northern Namibia. No additional specimens are known to have been collected beyond the type series. Herein, we report on eight specimens discovered at a second site near the original locality. DNA from four beetles was used to determine the phylogenetic placement of O. brainei among congeners, based on sequence data from one nuclear (histone III) and two mitochondrial (cox1, cox2) genes. Maximum likelihood analysis identifies O. brainei as a member of the ‘white’ Onymacris clade, in which it forms a strongly supported subclade with O. bicolor and O. marginipennis. More broadly, its phylogenetic placement augments previous molecular results that revealed a sister taxon relationship between the ‘white’ Onymacris and a second genus, Physadesmia. The paraphyly of Onymacris with respect to Physadesmia highlights a need for nomenclatural change, but revision should await acquisition of genetic data for the few species outstanding in both genera.
Open Access
Covariates of Identified Stress and Depression among Seasonal Farmworkers
(2017) Chaney, Beth H.; Torres, Essie
Many noted difficulties of farmworker life result in increased risk for stress and depression. To date, limited research has focused primarily on seasonal farmworkers; much of the prior research examines migrant farmworkers or both groups collectively. This study aims to: (1) describe levels of stress and depression among a sample of seasonal farmworkers; and (2) identify if covariates (age, gender, marital status, education level, years of residency, problems obtaining healthcare due to documentation, language barriers, transportation, costs, medical insurance, and stress level) are significant predictors of depressive symptoms. Survey data were collected from 150 Latino seasonal farmworkers. A hierarchical binary logistic regression was conducted to identify significant covariates. The results indicated that the only statistically significant covariates were health insurance coverage (p = 0.025) and stress (p = 0.008). Those farmworkers without health insurance were 1.8 times more likely than those with health insurance to possess depressive symptoms, while those demonstrating higher stress levels were over 7 times more likely to demonstrate symptoms of depression. The implications of the results are discussed in the manuscript.
Open Access
Quantitative analysis and comparison of 3D morphology between viable and apoptotic MCF-7 breast cancer cells and characterization of nuclear fragmentation
(2017-09-08) Wen, Yuhua; Chen, Zhan; Lu, Jianfen; Ables, Elizabeth Tweedie; Scemama, Jean-Luc; Yang, Liv. V.; Lu, Jun Q.; Hu, Xin-Hua
Morphological changes in apoptotic cells provide essential markers for defining and detection of apoptosis as a fundamental mechanism of cell death. Among these changes, the nuclear fragmentation and condensation have been regarded as the important markers but quantitative characterization of these changes is yet to be achieved. We have acquired confocal image stacks of 206 viable and apoptotic MCF-7 cells stained by three fluorescent dyes. Three-dimensional (3D) parameters were extracted to quantify and compare their differences in morphology. To analyze nuclear fragmentation, a new method has been developed to determine clustering of nuclear voxels in the reconstructed cells due to fluorescence intensity changes in nuclei of apoptotic cells. The results of these studies reveal that the 3D morphological changes in cytoplasm and nuclear membranes in apoptotic cells provide sensitive targets for label-free detection and staging of apoptosis. Furthermore, the clustering analysis and morphological data on nuclear fragmentation are highly useful for derivation of optical cell models and simulation of diffraction images to investigate light scattering by early apoptotic cells, which can lead to future development of label-free and rapid methods of apoptosis assay based on cell morphology.
Open Access
Adaptive responses to salinity stress across multiple life stages in anuran amphibians
(2017-08-01) Albecker, Molly; McCoy, Michael W.
Background In many regions, freshwater wetlands are increasing in salinity at rates exceeding historic levels. Some freshwater organisms, like amphibians, may be able to adapt and persist in salt-contaminated wetlands by developing salt tolerance. Yet adaptive responses may be more challenging for organisms with complex life histories, because the same environmental stressor can require responses across different ontogenetic stages. Here we investigated responses to salinity in anuran amphibians: a common, freshwater taxon with a complex life cycle. We conducted a meta-analysis to define how the lethality of saltwater exposure changes across multiple life stages, surveyed wetlands in a coastal region experiencing progressive salinization for the presence of anurans, and used common garden experiments to investigate whether chronic salt exposure alters responses in three sequential life stages (reproductive, egg, and tadpole life stages) in Hyla cinerea, a species repeatedly observed in saline wetlands. Results Meta-analysis revealed differential vulnerability to salt stress across life stages with the egg stage as the most salt-sensitive. Field surveys revealed that 25% of the species known to occur in the focal region were detected in salt-intruded habitats. Remarkably, Hyla cinerea was found in large abundances in multiple wetlands with salinity concentrations 450% higher than the tadpole-stage LC50. Common garden experiments showed that coastal (chronically salt exposed) populations of H. cinerea lay more eggs, have higher hatching success, and greater tadpole survival in higher salinities compared to inland (salt naïve) populations. Conclusions Collectively, our data suggest that some species of anuran amphibians have divergent and adaptive responses to salt exposure across populations and across different life stages. We propose that anuran amphibians may be a novel and amenable natural model system for empirical explorations of adaptive responses to environmental change.
Open Access
Reliability and Validity of the Telephone-Based eHealth Literacy Scale Among Older Adults: Cross-Sectional Survey
(2017) Stellefson, Michael; Paige, Samantha R.; Tennant, Bethany; Alber, Julia M.; Chaney, Beth H.; Chaney, J. Don; Grossman, Suzanne
Background: Only a handful of studies have examined reliability and validity evidence of scores produced by the 8-item eHealth literacy Scale (eHEALS) among older adults. Older adults are generally more comfortable responding to survey items when asked by a real person rather than by completing self-administered paper-and-pencil or online questionnaires. However, no studies have explored the psychometrics of this scale when administered to older adults over the telephone. Objective: The objective of our study was to examine the reliability and internal structure of eHEALS data collected from older adults aged 50 years or older responding to items over the telephone. Methods: Respondents (N=283) completed eHEALS as part of a cross-sectional landline telephone survey. Exploratory structural equation modeling (E-SEM) analyses examined model fit of eHEALS scores with 1-, 2-, and 3-factor structures. Subsequent analyses based on the partial credit model explored the internal structure of eHEALS data. Results: Compared with 1- and 2-factor models, the 3-factor eHEALS structure showed the best global E-SEM model fit indices (root mean square error of approximation=.07; comparative fit index=1.0; Tucker-Lewis index=1.0). Nonetheless, the 3 factors were highly correlated (r range .36 to .65). Item analyses revealed that eHEALS items 2 through 5 were overfit to a minor degree (mean square infit/outfit values <1.0; t statistics less than –2.0), but the internal structure of Likert scale response options functioned as expected. Overfitting eHEALS items (2-5) displayed a similar degree of information for respondents at similar points on the latent continuum. Test information curves suggested that eHEALS may capture more information about older adults at the higher end of the latent continuum (ie, those with high eHealth literacy) than at the lower end of the continuum (ie, those with low eHealth literacy). Item reliability (value=.92) and item separation (value=11.31) estimates indicated that eHEALS responses were reliable and stable. Conclusions: Results support administering eHEALS over the telephone when surveying older adults regarding their use of the Internet for health information. eHEALS scores best captured 3 factors (or subscales) to measure eHealth literacy in older adults; however, statistically significant correlations between these 3 factors suggest an overarching unidimensional structure with 3 underlying dimensions. As older adults continue to use the Internet more frequently to find and evaluate health information, it will be important to consider modifying the original eHEALS to adequately measure societal shifts in online health information seeking among aging populations.
Open Access
Baseline Assessment of a Healthy Corner Store Initiative: Associations between Food Store Environments, Shopping Patterns, Customer Purchases, and Dietary Intake in Eastern North Carolina
(2017) Jilcott Pitts, Stephanie Bell; Wu, Qiang; Laska, Melissa Nelson; Grinchak, Taras; McGuirt, Jared T.; Haynes-Maslow, Lindsey; Bell, Ronny A.; Ammerman, Alice S.; Truesdale, Kimberly P.
Open Access
A second locality for the Namib darkling beetle Onymacris brainei (Tenebrionidae, Coleoptera) and first report on its molecular phylogenetic placement
(2017) Lamb, Trip; Marais, Eugene; Bond, Jason E.
Onymacris brainei Penrith, 1984 – the most recent species of Onymacris to be described – is known only from its type locality in the Namib Desert, adjacent to the Cunene River in northern Namibia. No additional specimens are known to have been collected beyond the type series. Herein, we report on eight specimens discovered at a second site near the original locality. DNA from four beetles was used to determine the phylogenetic placement of O. brainei among congeners, based on sequence data from one nuclear (histone III) and two mitochondrial (cox1, cox2) genes. Maximum likelihood analysis identifies O. brainei as a member of the ‘white’ Onymacris clade, in which it forms a strongly supported subclade with O. bicolor and O. marginipennis. More broadly, its phylogenetic placement augments previous molecular results that revealed a sister taxon relationship between the ‘white’ Onymacris and a second genus, Physadesmia. The paraphyly of Onymacris with respect to Physadesmia highlights a need for nomenclatural change, but revision should await acquisition of genetic data for the few species outstanding in both genera.
Open Access
Responding to health and social needs of aging Latinos in new-growth communities: a qualitative study
(2017) Mathews, Holly F.; Torres, Essie; Lea, C. Suzanne; Larson, Kim L.
Background The development of new-growth communities of Latino immigrants in southern states has challenged the traditional health and social service infrastructure. An interprofessional team of service providers, Latino leaders, and university faculty partnered to establish linkages with the Latino community and providers serving aging adults and to explore the health and social needs of aging Latinos residing in a rural region. Methods A qualitative descriptive study was conducted through a community-university partnership, the Aging Latino Research Team (ALRT). Data were generated from nine focus groups and 15 key informant interviews with Latino and non-Latino community members and service providers in rural, eastern North Carolina (ENC). Results Thematic analysis was used to identify common patterns and form recommendations for future research and programs. Themes common to Latino participants were: “We are put off to one side”; “If I can't work, I can't survive”; and “Without documents, you are no one.” Themes common to non-Latino participants were: “Older Latinos are not well served”; “Older Latinos are invisible”; “Older Latinos are undocumented and afraid”; and “Older Latinos are wandering the highway”. Conclusion A major finding of this research was the extent to which discrepancies in perceptions between Latino participants and non-Latino participants exist. These discrepancies revealed ethnic stereotyping and cultural insensitivity as major barriers in access to care.
Open Access
The effects of elevated temperature and dissolved ρCO2 on a marine foundation species
(2017-04) Speights, Cori J.; Silliman, Brian R.; McCoy, Michael W.
Understanding how climate change and other environmental stressors will affect species is a fundamental concern of modern ecology. Indeed, numerous studies have documented how climate stressors affect species distributions and population persistence. However, relatively few studies have investigated how multiple climate stressors might affect species. In this study, we investigate the impacts of how two climate change factors affect an important foundation species. Specifically, we tested how ocean acidification from dissolution of CO2 and increased sea surface temperatures affect multiple characteristics of juvenile eastern oysters (Crassostrea virginica). We found strong impacts of each stressor, but no interaction between the two. Simulated warming to mimic heat stressed summers reduced oyster growth, survival, and filtration rates. Additionally, we found that CO2-induced acidification reduced strength of oyster shells, which could potentially facilitate crab predation. As past studies have detected few impacts of these stressors on adult oysters, these results indicate that early life stages of calcareous marine organisms may be more susceptible to effects of ocean acidification and global warming. Overall, these data show that predicted changes in temperature and CO2 can differentially influence direct effects on individual species, which could have important implications for the nature of their trophic interactions.
Open Access
A possible dose–response association between distance to farmers’ markets and roadside produce stands, frequency of shopping, fruit and vegetable consumption, and body mass index among customers in the Southern United States
(2017-01) Jilcott Pitts, Stephanie Bell; Hinkley, Jedediah; Wu, Qiang; McGuirt, Jared T.; Lyonnais, Mary Jane; Rafferty, Ann P.; Whitt, Olivia R.; Winterbauer, Nancy L.; Phillips, Lisa
Background: The association between farmers’ market characteristics and consumer shopping habits remains unclear. Our objective was to examine associations among distance to farmers’ markets, amenities within farmers’ markets, frequency of farmers’ market shopping, fruit and vegetable consumption, and body mass index (BMI). We hypothesized that the relationship between frequency of farmers’ market shopping and BMI would be mediated by fruit and vegetable consumption. Methods: In 15 farmers’ markets in northeastern North Carolina, July–September 2015, we conducted a crosssectional survey among 263 farmers’ market customers (199 provided complete address data) and conducted farmers’ market audits. To participate, customers had to be over 18 years of age, and English speaking. Dependent variables included farmers’ market shopping frequency, fruit and vegetable consumption, and BMI. Analysis of variance, adjusted multinomial logistic regression, Poisson regression, and linear regression models, adjusted for age, race, sex, and education, were used to examine associations between distance to farmers’ markets, amenities within farmers’ markets, frequency of farmers’ market shopping, fruit and vegetable consumption, and BMI. Results: Those who reported shopping at farmers’ markets a few times per year or less reported consuming 4.4 (standard deviation = 1.7) daily servings of fruits and vegetables, and those who reported shopping 2 or more times per week reported consuming 5.5 (2.2) daily servings. There was no association between farmers’ market amenities, and shopping frequency or fruit and vegetable consumption. Those who shopped 2 or more times per week had a statistically significantly lower BMI than those who shopped less frequently. There was no evidence of mediation of the relationship between frequency of shopping and BMI by fruit and vegetable consumption. Conclusions: More work should be done to understand factors within farmers’ markets that encourage fruit and vegetable purchases.