Advisor | Anderson , Eric | |
Advisor | Spuches , Anne | |
Author | Oliveira, Luis Felipe | |
Date Accessioned | 2023-07-13T16:48:47Z | |
Date Available | 2023-07-13T16:48:47Z | |
Date Created | 2023-05 | |
Date of Issue | 2023-04-26 | |
xmlui.metadata.dc.date.submitted | May 2023 | |
Identifier (URI) | http://hdl.handle.net/10342/12966 | |
Description | The purpose of this experimentation was to optimize the purification methodology for Human Cardiac Troponin C (HcTnC). This was done by replacing the old methodology of the size exclusion column and ion exchange column, with a phenyl sepharose column. This increased the protein concentration from 0.095 mg/mL to 0.251 mg/mL. This is important because it allows HcTnC to be purified faster with a higher yield, so more experimentation can be conducted. Further experimentation should be done to optimize the elution gradient as well as optimize the protein induction. | |
Mimetype | application/pdf | |
Publisher | East Carolina University | |
Subject | Chromatography, Biology, Biochemistry, Protein, Purification, FPLC, | |
Title | EXPRESSION AND PURIFICATION OF THE REGULATORY DOMAIN OF HUMAN CARDIAC TROPONIN C (HCTNC): A PROTEIN METHOD OPTIMIZATION STUDY | |
Type | Honors Thesis | |
xmlui.metadata.dc.date.updated | 2023-06-30T13:45:09Z | |
Department | Biology | |
xmlui.metadata.dc.degree.name | BS | |
xmlui.metadata.dc.degree.level | Undergraduate | |
xmlui.metadata.dc.degree.discipline | Biology | |
xmlui.metadata.dc.degree.grantor | East Carolina University | |
xmlui.metadata.dc.degree.department | Biology | |
xmlui.metadata.dc.access.option | Open Access | |
xmlui.metadata.dc.type.material | text | |