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    Kinetics of Binding of Caldesmon to Actin

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    Author
    Chalovich, Joseph M.; Chen, Yi-Der; Dudek, Ronald W.; Hai, Luo
    Abstract
    The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan actin. The concentration dependence of the observed rate of caldesmon-actin binding was analyzed to a first approximation as a single-step reaction using a Monte Carlo simulation. The derived association and dissociation rates were 107 M-1 s-1 and 18.2 s-1, respectively. Smooth muscle tropomyosin enhances the binding of caldesmon to actin, and this was found to be due to a reduction in the rate of dissociation to 6.3 s -1. There is no evidence from this study for a different mechanism of binding in the presence of tropomyosin. The fluorescence changes that occurred with the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or actin-tropomyosin were reversed by the addition of myosin subfragment 1 as predicted by a competitive binding mechanism. Originally published in the Journal of Biological Chemistry, Vol. 270, No. 17, 1995.
    URI
    http://hdl.handle.net/10342/2994
    Subject
     Caldesmon interactions; Flourescence changes in labeled molecules; Actin binding 
    Date
    1995-04-28
    Citation:
    APA:
    Chalovich, Joseph M., & Chen, Yi-Der, & Dudek, Ronald W., & Hai, Luo. (April 1995). Kinetics of Binding of Caldesmon to Actin. , (. Retrieved from http://hdl.handle.net/10342/2994

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    MLA:
    Chalovich, Joseph M., and Chen, Yi-Der, and Dudek, Ronald W., and Hai, Luo. "Kinetics of Binding of Caldesmon to Actin". . . (.), April 1995. February 26, 2021. http://hdl.handle.net/10342/2994.
    Chicago:
    Chalovich, Joseph M. and Chen, Yi-Der and Dudek, Ronald W. and Hai, Luo, "Kinetics of Binding of Caldesmon to Actin,"  , no. (April 1995), http://hdl.handle.net/10342/2994 (accessed February 26, 2021).
    AMA:
    Chalovich, Joseph M., Chen, Yi-Der, Dudek, Ronald W., Hai, Luo. Kinetics of Binding of Caldesmon to Actin. . April 1995; () . http://hdl.handle.net/10342/2994. Accessed February 26, 2021.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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