Kinetics of Binding of Caldesmon to Actin
Author
Chalovich, Joseph; Chen, Yi-Der; Dudek, Ronald W.; Hai, Luo
Abstract
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan actin. The concentration dependence of the observed rate of caldesmon-actin binding was analyzed to a first approximation as a single-step reaction using a Monte Carlo simulation. The derived association and dissociation rates were 107 M-1 s-1 and 18.2 s-1, respectively. Smooth muscle tropomyosin enhances the binding of caldesmon to actin, and this was found to be due to a reduction in the rate of dissociation to 6.3 s -1. There is no evidence from this study for a different mechanism of binding in the presence of tropomyosin. The fluorescence changes that occurred with the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or actin-tropomyosin were reversed by the addition of myosin subfragment 1 as predicted by a competitive binding mechanism. Originally published in the Journal of Biological Chemistry, Vol. 270, No. 17, 1995.
Date
1995-04-28
Citation:
APA:
Chalovich, Joseph, & Chen, Yi-Der, & Dudek, Ronald W., & Hai, Luo. (April 1995).
Kinetics of Binding of Caldesmon to Actin.
Journal of Biological Chemistry,
270(17),
9911-
9916. Retrieved from
http://hdl.handle.net/10342/2994
MLA:
Chalovich, Joseph, and Chen, Yi-Der, and Dudek, Ronald W., and Hai, Luo.
"Kinetics of Binding of Caldesmon to Actin". Journal of Biological Chemistry.
270:17. (9911-9916),
April 1995.
November 29, 2023.
http://hdl.handle.net/10342/2994.
Chicago:
Chalovich, Joseph and Chen, Yi-Der and Dudek, Ronald W. and Hai, Luo,
"Kinetics of Binding of Caldesmon to Actin," Journal of Biological Chemistry 270, no.
17 (April 1995),
http://hdl.handle.net/10342/2994 (accessed
November 29, 2023).
AMA:
Chalovich, Joseph, Chen, Yi-Der, Dudek, Ronald W., Hai, Luo.
Kinetics of Binding of Caldesmon to Actin. Journal of Biological Chemistry.
April 1995;
270(17):
9911-9916.
http://hdl.handle.net/10342/2994. Accessed
November 29, 2023.
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Publisher
East Carolina University