Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes
Lee, Tung-Kwang; Wang, Weidong; O'Brien, Kevin F.; Roberta, Johnke; Allison, Ron R.; Diaz, Angelica; Wang, Tao
To explore the radioprotective effect of a standardized North American ginseng extract (NAGE) on human peripheral blood lymphocytes (PBL), a micronuclei (MN) assay was conducted in PBL obtained from 12 volunteers. NAGE (50â 1000 Âµg/mL) and WR-1065 (1 mm and 3 mm) were applied to PBL cultures at 0 h and 90 min post-irradiation. It was found that (1) the baseline MN yield of PBL ranged from 14.4 Â± 1.5 to 15.9 Â± 1.5 per 1000 binucleated cells (p > 0.05); after irradiation (1 Gy and 2 Gy), the MN yield increased sharply; (2) MN yields declined with increasing concentrations of NAGE and WR-1065. Even at 90 min post-irradiation of 1 Gy, the maximum level of MN reduction rate caused by NAGE and WR-1065 was 53.8% and 59.2%, respectively; after 2 Gy irradiation, it was 37.3% and 42%, respectively; (3) the MN distribution in PBL followed a non-Poisson distribution in all cases; and (4) both NAGE and WR-1065 showed no significant effect on the proliferation index of lymphocytes. The results indicate that NAGE is a relatively non-toxic natural product, which can be administered as a dietary supplement and has the potential to be a radiation countermeasure. Originally published Phytotherapy Research Vol. 22, No. 12 2008.
Lee, Tung-Kwang, & Wang, Weidong, & O'Brien, Kevin F., & Roberta, Johnke, & Allison, Ron R., & Diaz, Angelica, & Wang, Tao. (December 2008). Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes. Phytotherapy Research, 22(12), 1614- 1622. Retrieved from http://hdl.handle.net/10342/3038
Lee, Tung-Kwang, and Wang, Weidong, and O'Brien, Kevin F., and Roberta, Johnke, and Allison, Ron R., and Diaz, Angelica, and Wang, Tao. "Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes". Phytotherapy Research. 22:12. (1614-1622), December 2008. September 22, 2023. http://hdl.handle.net/10342/3038.
Lee, Tung-Kwang and Wang, Weidong and O'Brien, Kevin F. and Roberta, Johnke and Allison, Ron R. and Diaz, Angelica and Wang, Tao, "Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes," Phytotherapy Research 22, no. 12 (December 2008), http://hdl.handle.net/10342/3038 (accessed September 22, 2023).
Lee, Tung-Kwang, Wang, Weidong, O'Brien, Kevin F., Roberta, Johnke, Allison, Ron R., Diaz, Angelica, Wang, Tao. Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes. Phytotherapy Research. December 2008; 22(12): 1614-1622. http://hdl.handle.net/10342/3038. Accessed September 22, 2023.
East Carolina University