Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes
Author
Lee, Tung-Kwang; Wang, Weidong; O'Brien, Kevin F.; Roberta, Johnke; Allison, Ron R.; Diaz, Angelica; Wang, Tao
Abstract
To explore the radioprotective effect of a standardized North American ginseng extract (NAGE) on human peripheral blood lymphocytes (PBL), a micronuclei (MN) assay was conducted in PBL obtained from 12 volunteers. NAGE (50â 1000 µg/mL) and WR-1065 (1 mm and 3 mm) were applied to PBL cultures at 0 h and 90 min post-irradiation. It was found that (1) the baseline MN yield of PBL ranged from 14.4 ± 1.5 to 15.9 ± 1.5 per 1000 binucleated cells (p > 0.05); after irradiation (1 Gy and 2 Gy), the MN yield increased sharply; (2) MN yields declined with increasing concentrations of NAGE and WR-1065. Even at 90 min post-irradiation of 1 Gy, the maximum level of MN reduction rate caused by NAGE and WR-1065 was 53.8% and 59.2%, respectively; after 2 Gy irradiation, it was 37.3% and 42%, respectively; (3) the MN distribution in PBL followed a non-Poisson distribution in all cases; and (4) both NAGE and WR-1065 showed no significant effect on the proliferation index of lymphocytes. The results indicate that NAGE is a relatively non-toxic natural product, which can be administered as a dietary supplement and has the potential to be a radiation countermeasure. Originally published Phytotherapy Research Vol. 22, No. 12 2008.
Subject
Date
2008-12
Citation:
APA:
Lee, Tung-Kwang, & Wang, Weidong, & O'Brien, Kevin F., & Roberta, Johnke, & Allison, Ron R., & Diaz, Angelica, & Wang, Tao. (December 2008).
Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes.
Phytotherapy Research,
22(12),
1614-
1622. Retrieved from
http://hdl.handle.net/10342/3038
MLA:
Lee, Tung-Kwang, and Wang, Weidong, and O'Brien, Kevin F., and Roberta, Johnke, and Allison, Ron R., and Diaz, Angelica, and Wang, Tao.
"Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes". Phytotherapy Research.
22:12. (1614-1622),
December 2008.
September 22, 2023.
http://hdl.handle.net/10342/3038.
Chicago:
Lee, Tung-Kwang and Wang, Weidong and O'Brien, Kevin F. and Roberta, Johnke and Allison, Ron R. and Diaz, Angelica and Wang, Tao,
"Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes," Phytotherapy Research 22, no.
12 (December 2008),
http://hdl.handle.net/10342/3038 (accessed
September 22, 2023).
AMA:
Lee, Tung-Kwang, Wang, Weidong, O'Brien, Kevin F., Roberta, Johnke, Allison, Ron R., Diaz, Angelica, Wang, Tao.
Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes. Phytotherapy Research.
December 2008;
22(12):
1614-1622.
http://hdl.handle.net/10342/3038. Accessed
September 22, 2023.
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Publisher
East Carolina University