• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Microbiology and Immunology
    • View Item
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Microbiology and Immunology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa

    Thumbnail
    View/ Open
    Regulation fo pseudomonas quinolone.pdf (302.6Kb)

    Show full item record
    Author
    Wade, Dana S.; Calfee, M. Worth; Rocha, Edson R.; Ling, Elizabeth A.; Engstrom, Elana; Coleman, James P.; Pesci, Everett C.
    Abstract
    Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in cystic fibrosis patients and is a major source of nosocomial infections. This bacterium controls many virulence factors by using two quorum-sensing systems, las and rhl. The las system is composed of the LasR regulator protein and its cell-to-cell signal, N-(3-oxododecanoyl) homoserine lactone, and the rhl system is composed of RhlR and the signal N-butyryl homoserine lactone. A third intercellular signal, the Pseudomonas quinolone signal (PQS; 2-heptyl-3-hydroxy-4-quinolone), also regulates numerous virulence factors. PQS synthesis requires the expression of multiple operons, one of which is pqsABCDE. Previous experiments showed that the transcription of this operon, and therefore PQS production, is negatively regulated by the rhl quorum-sensing system and positively regulated by the las quorum-sensing system and PqsR (also known as MvfR), a LysR-type transcriptional regulator protein. With the use of DNA mobility shift assays and beta-galactosidase reporter fusions, we have studied the regulation of pqsR and its relationship to pqsA, lasR, and rhlR. We show that PqsR binds the promoter of pqsA and that this binding increases dramatically in the presence of PQS, implying that PQS acts as a coinducer for PqsR. We have also mapped the transcriptional start site for pqsR and found that the transcription of pqsR is positively regulated by lasR and negatively regulated by rhlR. These results suggest that a regulatory chain occurs where pqsR is under the control of LasR and RhlR and where PqsR in turn controls pqsABCDE, which is required for the production of PQS. Originally published Journal of Bacteriology, Vol. 187, No. 13, July 2005
    URI
    http://hdl.handle.net/10342/3109
    Subject
     Quinolone signal synthesis; Chronic lung infections; Cystic Fibrosis 
    Date
    2005-07
    Citation:
    APA:
    Wade, Dana S., & Calfee, M. Worth, & Rocha, Edson R., & Ling, Elizabeth A., & Engstrom, Elana, & Coleman, James P., & Pesci, Everett C.. (July 2005). Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa. , (. Retrieved from http://hdl.handle.net/10342/3109

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Wade, Dana S., and Calfee, M. Worth, and Rocha, Edson R., and Ling, Elizabeth A., and Engstrom, Elana, and Coleman, James P., and Pesci, Everett C.. "Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa". . . (.), July 2005. May 22, 2022. http://hdl.handle.net/10342/3109.
    Chicago:
    Wade, Dana S. and Calfee, M. Worth and Rocha, Edson R. and Ling, Elizabeth A. and Engstrom, Elana and Coleman, James P. and Pesci, Everett C., "Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa,"  , no. (July 2005), http://hdl.handle.net/10342/3109 (accessed May 22, 2022).
    AMA:
    Wade, Dana S., Calfee, M. Worth, Rocha, Edson R., Ling, Elizabeth A., Engstrom, Elana, Coleman, James P., Pesci, Everett C.. Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa. . July 2005; () . http://hdl.handle.net/10342/3109. Accessed May 22, 2022.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback