Absence of unsaturated fatty acid synthesis in murine T lymphocytes.
Buttke, Thomas M.; Van Cleave, Steven; Steelman, Linda; McCubrey, James A.
Stearic acid is toxic for T lymphocytes in vitro but has little effect on B lymphocytes. To investigate the molecular basis for this difference, purified murine T and B lymphocytes were compared for their abilities to incorporate and metabolize stearic acid. Unstimulated T and B cells incorporated identical amounts of stearic acid into six different phospholipids and four neutral lipids. After mitogen stimulation, fatty acid uptake was increased in both lymphocyte types, but cell-specific differences were seen in the distribution of stearic acid among the various cellular lipids. Doses of stearic acid that selectively inhibited T-cell proliferation resulted in a 5-fold greater accumulation of distearoylphosphatidylcholine in T cells than in B cells. Whereas T cells did not desaturate the exogenously derived stearic acid, up to 25% of the saturated fatty acid was converted to oleic acid in B cells. These findings suggested a deficiency of stearoyl-CoA desaturase (acyl-CoA, hydrogen-donor:oxygen oxidoreductase, EC 188.8.131.52) activity in T cells, which was confirmed by subsequent studies. Cell-free extracts from B cells displayed nearly 20-fold more stearoyl- CoA desaturase activity than T-cell extracts, and the level of stearoyl-CoA desaturase mRNA was 30-fold higher in B cells. Collectively, our data indicate that murine T cells are deficient in unsaturated fatty acid synthesis. The deficiency of stearoyl- CoA desaturase in T cells may represent the basis for the differing sensitivities of T and B lymphocytes to inhibition by saturated fatty acids. Originally published Proceedings of the National Academy of Sciences, Vol. 86, No. 16, Aug 1989
Buttke, Thomas M., & Van Cleave, Steven, & Steelman, Linda, & McCubrey, James A.. (August 1989). Absence of unsaturated fatty acid synthesis in murine T lymphocytes.. Proceedings of the National Academy of Sciences, 86(16), 6133- 6137. Retrieved from http://hdl.handle.net/10342/3237
Buttke, Thomas M., and Van Cleave, Steven, and Steelman, Linda, and McCubrey, James A.. "Absence of unsaturated fatty acid synthesis in murine T lymphocytes.". Proceedings of the National Academy of Sciences. 86:16. (6133-6137), August 1989. November 29, 2023. http://hdl.handle.net/10342/3237.
Buttke, Thomas M. and Van Cleave, Steven and Steelman, Linda and McCubrey, James A., "Absence of unsaturated fatty acid synthesis in murine T lymphocytes.," Proceedings of the National Academy of Sciences 86, no. 16 (August 1989), http://hdl.handle.net/10342/3237 (accessed November 29, 2023).
Buttke, Thomas M., Van Cleave, Steven, Steelman, Linda, McCubrey, James A.. Absence of unsaturated fatty acid synthesis in murine T lymphocytes.. Proceedings of the National Academy of Sciences. August 1989; 86(16): 6133-6137. http://hdl.handle.net/10342/3237. Accessed November 29, 2023.
East Carolina University