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    Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing

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    Author
    Filippova, Elena M.; Monteleone, Denise C.; Trunk, John G.; Sutherland, Betsy M.; Quake, Stephen R.; Sutherland, John C.
    Abstract
    Fluorescence from a single DNA molecule passing through a laser beam is proportional to the size (contour length) of the molecule, and molecules of different sizes can be counted with equal efficiencies. Single-molecule fluorescence can thus determine the average length of the molecules in a sample and hence the frequency of double-strand breaks induced by various treatments. Ionizing radiation-induced frank double-strand breaks can thus be quantified by single-molecule sizing. Moreover, multiple classes of clustered damages involving damaged bases and abasic sites, alone or in combination with frank single-strand breaks, can be quantified by converting them to double-strand breaks by chemical or enzymatic treatments. For a given size range of DNA molecules, single-molecule sizing is as or more sensitive than gel electrophoresis, and requires several orders-of-magnitude less DNA to determine damage levels. Originally published Biophysical Journal, Vol. 84, No. 2, Feb 2003
    URI
    http://hdl.handle.net/10342/3265
    Subject
     Laser fluorescence sizing; DNA; Double-strand breaks; Cluster damage 
    Date
    2003-02
    Citation:
    APA:
    Filippova, Elena M., & Monteleone, Denise C., & Trunk, John G., & Sutherland, Betsy M., & Quake, Stephen R., & Sutherland, John C.. (February 2003). Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing. Biophysical Journal, 84(2), 1281- 1290. Retrieved from http://hdl.handle.net/10342/3265

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Filippova, Elena M., and Monteleone, Denise C., and Trunk, John G., and Sutherland, Betsy M., and Quake, Stephen R., and Sutherland, John C.. "Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing". Biophysical Journal. 84:2. (1281-1290), February 2003. October 03, 2023. http://hdl.handle.net/10342/3265.
    Chicago:
    Filippova, Elena M. and Monteleone, Denise C. and Trunk, John G. and Sutherland, Betsy M. and Quake, Stephen R. and Sutherland, John C., "Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing," Biophysical Journal 84, no. 2 (February 2003), http://hdl.handle.net/10342/3265 (accessed October 03, 2023).
    AMA:
    Filippova, Elena M., Monteleone, Denise C., Trunk, John G., Sutherland, Betsy M., Quake, Stephen R., Sutherland, John C.. Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing. Biophysical Journal. February 2003; 84(2): 1281-1290. http://hdl.handle.net/10342/3265. Accessed October 03, 2023.
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    East Carolina University

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