Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing
Author
Filippova, Elena M.; Monteleone, Denise C.; Trunk, John G.; Sutherland, Betsy M.; Quake, Stephen R.; Sutherland, John C.
Abstract
Fluorescence from a single DNA molecule passing through a laser beam is proportional to the size (contour length) of the molecule, and molecules of different sizes can be counted with equal efficiencies. Single-molecule fluorescence can thus determine the average length of the molecules in a sample and hence the frequency of double-strand breaks induced by various treatments. Ionizing radiation-induced frank double-strand breaks can thus be quantified by single-molecule sizing. Moreover, multiple classes of clustered damages involving damaged bases and abasic sites, alone or in combination with frank single-strand breaks, can be quantified by converting them to double-strand breaks by chemical or enzymatic treatments. For a given size range of DNA molecules, single-molecule sizing is as or more sensitive than gel electrophoresis, and requires several orders-of-magnitude less DNA to determine damage levels. Originally published Biophysical Journal, Vol. 84, No. 2, Feb 2003
Date
2003-02
Citation:
APA:
Filippova, Elena M., & Monteleone, Denise C., & Trunk, John G., & Sutherland, Betsy M., & Quake, Stephen R., & Sutherland, John C.. (February 2003).
Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing.
Biophysical Journal,
84(2),
1281-
1290. Retrieved from
http://hdl.handle.net/10342/3265
MLA:
Filippova, Elena M., and Monteleone, Denise C., and Trunk, John G., and Sutherland, Betsy M., and Quake, Stephen R., and Sutherland, John C..
"Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing". Biophysical Journal.
84:2. (1281-1290),
February 2003.
October 03, 2023.
http://hdl.handle.net/10342/3265.
Chicago:
Filippova, Elena M. and Monteleone, Denise C. and Trunk, John G. and Sutherland, Betsy M. and Quake, Stephen R. and Sutherland, John C.,
"Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing," Biophysical Journal 84, no.
2 (February 2003),
http://hdl.handle.net/10342/3265 (accessed
October 03, 2023).
AMA:
Filippova, Elena M., Monteleone, Denise C., Trunk, John G., Sutherland, Betsy M., Quake, Stephen R., Sutherland, John C..
Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing. Biophysical Journal.
February 2003;
84(2):
1281-1290.
http://hdl.handle.net/10342/3265. Accessed
October 03, 2023.
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Publisher
East Carolina University