Author | Jones, Heath | en_US |
Author | Carver, Melinda | en_US |
Author | Pekala, Phillip H. | en_US |
Date Accessioned | 2011-04-13T20:53:41Z | en_US |
Date Accessioned | 2011-05-17T01:27:02Z | |
Date Available | 2011-04-13T20:53:41Z | en_US |
Date Available | 2011-05-17T01:27:02Z | |
Date of Issue | 2007-03-30 | en_US |
Identifier (Citation) | Biochemical and Biophysical Research Communications; 355:1 p. 217-220 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/3315 | en_US |
Description | HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. Once bound to the mRNA, HuR is recognized by adapter proteins which then facilitate nuclear export of the complex. In the cytosol HuR is thought to function to control stability and translation of its ligand message. In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. However within 30 min of exposure to
the differentiation stimulus, the HuR content in the cytosol increases consistent with HuR regulating the availability of relevant mRNAs for translation. Using in vitro RNA gel shifts, we have demonstrated that the C/EBPß message is a ligand for HuR and that the single binding site is an adenylate-uridylate rich element in the 3'untranslated region. Originally published Biochemical and Biophysical Research Communications, Vol. 355, No. 1, Mar 2007 | en_US |
Language | en_US | en_US |
Publisher | East Carolina University | en_US |
Related URI | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4MYMJJF-8&_user=10&_coverDate=03%2F30%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=41beeded65d947ad9b1f39f98e5c276f&searchtype=a | en_US |
Rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
Subject | CCAAT enhancer binding protein beta | en_US |
Subject | HuR | en_US |
Subject | Elav | en_US |
Subject | 3T3-L1 | en_US |
Subject | Adipocytes | en_US |
Subject | Differentiation | en_US |
Title | HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes | en_US |
Type | Article | en_US |
Identifier (PMID) | PMC1839008 | en_US |
Identifier (DOI) | 10.1016/j.bbrc.2007.01.136 | |
Journal Name | Biochemical and Biophysical Research Communications | |
Journal Volume | 355 | |
Journal Issue | 1 | |
Article Pages | 217-220 | |