Identification of E2F1 as a positive transcriptional regulator for delta-catenin
Kim, Kwonseop; Oh, Minsoo; Ki, Hyunkyoung; Wang, Tao; Bareiss, Sonja; Fini, M. Elizabeth; Li, Dawei; Lu, Qun
Delta-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate delta-catenin expression in cancer. Using a human delta-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect delta-catenin transcription. Among beta-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased delta-catenin-luciferase activities while beta-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of delta-catenin-luciferase activities induced by E2F1 but did not interact with delta-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of delta-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on delta-catenin expression were observed only in human cancer cells expressing abundant endogenous delta-catenin. These studies identify E2F1 as a positive transcriptional regulator for delta-catenin, but further suggest the presence of strong negative regulator(s) for delta-catenin in prostate cancer cells with minimal endogenous delta-catenin expression. Originally published Biochem Biophys REs Commun, Vol. 369, No. 2, May 2008
Kim, Kwonseop, & Oh, Minsoo, & Ki, Hyunkyoung, & Wang, Tao, & Bareiss, Sonja, & Fini, M. Elizabeth, & Li, Dawei, & Lu, Qun. (May 2008). Identification of E2F1 as a positive transcriptional regulator for delta-catenin. 414-420, (369:2), p.. Retrieved from http://hdl.handle.net/10342/3324
Kim, Kwonseop, and Oh, Minsoo, and Ki, Hyunkyoung, and Wang, Tao, and Bareiss, Sonja, and Fini, M. Elizabeth, and Li, Dawei, and Lu, Qun. "Identification of E2F1 as a positive transcriptional regulator for delta-catenin". 414-420. 369:2. (.), May 2008. April 10, 2020. http://hdl.handle.net/10342/3324.
Kim, Kwonseop and Oh, Minsoo and Ki, Hyunkyoung and Wang, Tao and Bareiss, Sonja and Fini, M. Elizabeth and Li, Dawei and Lu, Qun, "Identification of E2F1 as a positive transcriptional regulator for delta-catenin," 414-420 369, no. 2 (May 2008), http://hdl.handle.net/10342/3324 (accessed April 10, 2020).
Kim, Kwonseop, Oh, Minsoo, Ki, Hyunkyoung, Wang, Tao, Bareiss, Sonja, Fini, M. Elizabeth, Li, Dawei, Lu, Qun. Identification of E2F1 as a positive transcriptional regulator for delta-catenin. 414-420. May 2008; 369(2) . http://hdl.handle.net/10342/3324. Accessed April 10, 2020.
East Carolina University