The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.

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Author
MacGregor, Carolyn H.; Arora, Shiwani K.; Hager, Paul W.; Dail, Mary Beth; Phibbs, Paul V. Jr.
Abstract
The gene (crc) responsible for catabolite repression control in Pseudomonas aeruginosa has been cloned and sequenced. Flanking the crc gene are genes encoding orotate phosphoribosyl transferase (pyrE) and RNase PH (rph). New crc mutants were constructed by disruption of the wild-type crc gene. The crc gene encodes an open reading frame of 259 amino acids with homology to the apurinic/apyrimidinic endonuclease family of DNA repair enzymes. However, crc mutants do not have a DNA repair phenotype, nor can the crc gene complement Escherichia coli DNA repair-deficient strains. The crc gene product was overexpressed in both P. aeruginosa and in E. coli, and the Crc protein was purified from both. The purified Crc proteins show neither apurinic/ apyrimidinic endonuclease nor exonuclease activity. Antibody to the purified Crc protein reacted with proteins of similar size in crude extracts from Pseudomonas putida and Pseudomonas fluorescens, suggesting a common mechanism of catabolite repression in these three species. Originally published Journal of Bacteriology, Vol. 178, No. 19, Oct 1996
URI
http://hdl.handle.net/10342/3346
Subject
 Pseudomonas aeruginosa; Crc gene; Catabolite repression 
Date
1996-10
Citation:
APA:
MacGregor, Carolyn H., & Arora, Shiwani K., & Hager, Paul W., & Dail, Mary Beth, & Phibbs, Paul V. Jr.. (October 1996). The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.. , (. Retrieved from http://hdl.handle.net/10342/3346

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MLA:
MacGregor, Carolyn H., and Arora, Shiwani K., and Hager, Paul W., and Dail, Mary Beth, and Phibbs, Paul V. Jr.. "The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.". . . (.), October 1996. August 10, 2020. http://hdl.handle.net/10342/3346.
Chicago:
MacGregor, Carolyn H. and Arora, Shiwani K. and Hager, Paul W. and Dail, Mary Beth and Phibbs, Paul V. Jr., "The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.,"  , no. (October 1996), http://hdl.handle.net/10342/3346 (accessed August 10, 2020).
AMA:
MacGregor, Carolyn H., Arora, Shiwani K., Hager, Paul W., Dail, Mary Beth, Phibbs, Paul V. Jr.. The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.. . October 1996; () . http://hdl.handle.net/10342/3346. Accessed August 10, 2020.
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East Carolina University