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    The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa

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    Author
    McKnight, Susan L.; Iglewski, Barbara H.; Pesci, Everett C.
    Abstract
    The opportunistic pathogen Pseudomonas aeruginosa uses intercellular signals to control the density-dependent expression of many virulence factors. The las and rhl quorum-sensing systems function, respectively, through the autoinducers N-(3-oxododecanoyl)-L-homoserine lactone and N-butyryl-L-homoserine lactone (C4- HSL), which are known to positively regulate the transcription of the elastase-encoding gene, lasB. Recently, we reported that a second type of intercellular signal is involved in lasB induction. This signal was identified as 2-heptyl-3-hydroxy-4-quinolone and designated the Pseudomonas quinolone signal (PQS). PQS was determined to be part of the quorum-sensing hierarchy since its production and bioactivity depended on the las and rhl quorum-sensing systems, respectively. In order to define the role of PQS in the P. aeruginosa quorumsensing cascade, lacZ gene fusions were used to determine the effect of PQS on the transcription of the quorum-sensing system genes lasR, lasI, rhlR, and rhlI. We found that in P. aeruginosa, PQS caused a major induction of rhlI*-lacZ and had lesser effects on the transcription of lasR*-lacZ and rhlR*-lacZ. We also observed that the transcription of both rhlI*-lacZ and lasB*-lacZ was cooperatively effected by C4-HSL and PQS. Additionally, we present data indicating that PQS was not produced maximally until cultures reached the late stationary phase of growth. Taken together, our results imply that PQS acts as a link between the las and rhl quorum-sensing systems and that this signal is not involved in sensing cell density. Originally published Journal of Bacteriology, Vol. 182, No. 10, May 2000
    URI
    http://hdl.handle.net/10342/3355
    Subject
     Pseudomonas aeruginosa; Quinolone signal; Las quorum sensing; Rhl quorum sensing 
    Date
    2000-05
    Citation:
    APA:
    McKnight, Susan L., & Iglewski, Barbara H., & Pesci, Everett C.. (May 2000). The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa. Journal of Bacteriology, 182(10), 2702- 2708. Retrieved from http://hdl.handle.net/10342/3355

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    McKnight, Susan L., and Iglewski, Barbara H., and Pesci, Everett C.. "The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa". Journal of Bacteriology. 182:10. (2702-2708), May 2000. September 26, 2023. http://hdl.handle.net/10342/3355.
    Chicago:
    McKnight, Susan L. and Iglewski, Barbara H. and Pesci, Everett C., "The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa," Journal of Bacteriology 182, no. 10 (May 2000), http://hdl.handle.net/10342/3355 (accessed September 26, 2023).
    AMA:
    McKnight, Susan L., Iglewski, Barbara H., Pesci, Everett C.. The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa. Journal of Bacteriology. May 2000; 182(10): 2702-2708. http://hdl.handle.net/10342/3355. Accessed September 26, 2023.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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