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    The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa

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    Author
    O'Toole, George A.; Gibbs, Karine A.; Hager, Paul W.; Phibbs, Paul V. Jr.; Kolter, Roberto
    Abstract
    The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite repression control (Crc) protein, which plays a role in the regulation of carbon metabolism, is necessary for biofilm formation in Pseudomonas aeruginosa. Using phase-contrast microscopy, we found that a crc mutant only makes a dispersed monolayer of cells on a plastic surface but does not develop the dense monolayer punctuated by microcolonies typical of the wild-type strain. This is a phenotype identical to that observed in mutants defective in type IV pilus biogenesis. Consistent with this observation, crc mutants are defective in type IV pilus-mediated twitching motility. We show that this defect in type IV pilus function is due (at least in part) to a decrease in pilA (pilin) transcription. We propose that nutritional cues are integrated by Crc as part of a signal transduction pathway that regulates biofilm development. Originally published Journal of Bacteriology, Vol. 182, No. 2, Jan 2000
    URI
    http://hdl.handle.net/10342/3373
    Subject
     Biofilms; Pseudomonas aeruginosa; Catabolite repression control 
    Date
    2000-01
    Citation:
    APA:
    O'Toole, George A., & Gibbs, Karine A., & Hager, Paul W., & Phibbs, Paul V. Jr., & Kolter, Roberto. (January 2000). The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa. Journal of Bacteriology, (182:2), p.425-431. Retrieved from http://hdl.handle.net/10342/3373

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    O'Toole, George A., and Gibbs, Karine A., and Hager, Paul W., and Phibbs, Paul V. Jr., and Kolter, Roberto. "The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa". Journal of Bacteriology. 182:2. (425-431.), January 2000. May 22, 2022. http://hdl.handle.net/10342/3373.
    Chicago:
    O'Toole, George A. and Gibbs, Karine A. and Hager, Paul W. and Phibbs, Paul V. Jr. and Kolter, Roberto, "The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa," Journal of Bacteriology 182, no. 2 (January 2000), http://hdl.handle.net/10342/3373 (accessed May 22, 2022).
    AMA:
    O'Toole, George A., Gibbs, Karine A., Hager, Paul W., Phibbs, Paul V. Jr., Kolter, Roberto. The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa. Journal of Bacteriology. January 2000; 182(2) 425-431. http://hdl.handle.net/10342/3373. Accessed May 22, 2022.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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