The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa
Author
O'Toole, George A.; Gibbs, Karine A.; Hager, Paul W.; Phibbs, Paul V. Jr.; Kolter, Roberto
Abstract
The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite repression control (Crc) protein, which plays a role in the regulation of carbon metabolism, is necessary for biofilm formation in Pseudomonas aeruginosa. Using phase-contrast microscopy, we found that a crc mutant only makes a dispersed monolayer of cells on a plastic surface but does not develop the dense monolayer punctuated by microcolonies typical of the wild-type strain. This is a phenotype identical to that observed in mutants defective in type IV pilus biogenesis. Consistent with this observation, crc mutants are defective in type IV pilus-mediated twitching motility. We show that this defect in type IV pilus function is due (at least in part) to a decrease in pilA (pilin) transcription. We propose that nutritional cues are integrated by Crc as part of a signal transduction pathway that regulates biofilm development. Originally published Journal of Bacteriology, Vol. 182, No. 2, Jan 2000
Date
2000-01
Citation:
APA:
O'Toole, George A., & Gibbs, Karine A., & Hager, Paul W., & Phibbs, Paul V. Jr., & Kolter, Roberto. (January 2000).
The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa.
Journal of Bacteriology,
182(2),
425-
431. Retrieved from
http://hdl.handle.net/10342/3373
MLA:
O'Toole, George A., and Gibbs, Karine A., and Hager, Paul W., and Phibbs, Paul V. Jr., and Kolter, Roberto.
"The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa". Journal of Bacteriology.
182:2. (425-431),
January 2000.
May 08, 2024.
http://hdl.handle.net/10342/3373.
Chicago:
O'Toole, George A. and Gibbs, Karine A. and Hager, Paul W. and Phibbs, Paul V. Jr. and Kolter, Roberto,
"The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa," Journal of Bacteriology 182, no.
2 (January 2000),
http://hdl.handle.net/10342/3373 (accessed
May 08, 2024).
AMA:
O'Toole, George A., Gibbs, Karine A., Hager, Paul W., Phibbs, Paul V. Jr., Kolter, Roberto.
The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa. Journal of Bacteriology.
January 2000;
182(2):
425-431.
http://hdl.handle.net/10342/3373. Accessed
May 08, 2024.
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Publisher
East Carolina University