• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Physiology
    • View Item
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Physiology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice

    Thumbnail
    View/ Open
    Alveolar macrophage priming intravenous.pdf (175.2Kb)

    Show full item record
    Author
    Shibata, Yoshimi; Foster, L. Ann; Metzger, James; Myrvik, Quentin N.
    Abstract
    Intravenous (i.v.) administration of phagocytosable chitin particles (1 to 10 mm) in C57BL/6 mice and SCID mice primed alveolar macrophages (Mf) within 3 days to yield up to a 50-fold increase in their oxidative burst when elicited in vitro with phorbol myristate acetate (PMA). C57BL/6 mice pretreated with monoclonal antibodies (MAbs) against mouse gamma interferon (IFN-g) or NK1.1 showed a markedly decreased level of alveolar Mf priming following injection of chitin particles. To confirm IFN-g production in vitro, spleen cells isolated from normal C57BL/6 mice and SCID mice were cultured with chitin particles. Significant IFN-g production was observed following stimulation with chitin but not with chitosan or latex beads. When spleen cells were treated with anti-NK1.1 MAb, IFN-g production was significantly inhibited. Another set of experiments showed that when C57BL/6 mice were pretreated i.v. with a small dose IFN-g, a higher level of priming was induced with not only phagocytosable chitin particles but also phagocytosable chitosan and even latex beads. Likewise, the spleen cell cultures preconditioned with IFN-g provided an up-regulation of IFN-g production by these phagocytosable particles. Taken together, the in vivo and in vitro results suggest that (i) the alveolar Mf priming mechanism is due, at least in part, to direct activation of Mf by IFN-g, which is produced by NK1.11 CD42 cells; (ii) IFN-g would have an autocrine-like effect on Mf and make them more responsive to particle priming; and (iii) phagocytosis of particulates, probably by a postmembrane event such as interiorization, appears to be important for the up-regulation of alveolar Mf priming and IFN-g production. Originally published Infection and Immunity, Vol. 65, No. 5, May 1997
    URI
    http://hdl.handle.net/10342/3404
    Subject
     Alveolar macrophages; Chitin particles; N-acetyl-D-glucosamine 
    Date
    1997-03
    Citation:
    APA:
    Shibata, Yoshimi, & Foster, L. Ann, & Metzger, James, & Myrvik, Quentin N.. (March 1997). Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice. Infection and Immunity, (65:5), p.1734-1741. Retrieved from http://hdl.handle.net/10342/3404

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Shibata, Yoshimi, and Foster, L. Ann, and Metzger, James, and Myrvik, Quentin N.. "Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice". Infection and Immunity. 65:5. (1734-1741.), March 1997. August 18, 2022. http://hdl.handle.net/10342/3404.
    Chicago:
    Shibata, Yoshimi and Foster, L. Ann and Metzger, James and Myrvik, Quentin N., "Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice," Infection and Immunity 65, no. 5 (March 1997), http://hdl.handle.net/10342/3404 (accessed August 18, 2022).
    AMA:
    Shibata, Yoshimi, Foster, L. Ann, Metzger, James, Myrvik, Quentin N.. Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice. Infection and Immunity. March 1997; 65(5) 1734-1741. http://hdl.handle.net/10342/3404. Accessed August 18, 2022.
    Collections
    • Physiology
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback