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    Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.

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    Author
    Armstrong, Sandra K.; Clements, Mark O.
    Abstract
    Iron acquisition by the gram-negative pathogens Bordetella bronchiseptica and Bordetella pertussis is thought to occur by hydroxamate siderophore-mediated transport as well as an apparently siderophore-independent process by which host transferrins bind to bacterial surface receptors. We constructed B. bronchiseptica mutants deficient in siderophore activity by insertional mutagenesis with miniTn5/LacZl. The mutants could be placed into four distinct complementation groups, as determined from cross-feeding assays which demonstrated restored siderophore synthesis. Mutants deficient in siderophore activity were BRM1, BRM6, and BRM9, exhibiting approximately 36 to 41% of wild-type siderophore levels, and BRM3 and BRM8, which appeared to produce very little or no detectable siderophore. Mutant BRM4 was found to be a leucine auxotroph, while mutants BRM2 and BRM7 could synthesize siderophore only in low-iron medium which was supplemented with various amino acids. Evaluation of all transcriptional fusions revealed an apparent lack of iron-regulated lacZ expression. Genomic regions flanking the transposable element in the siderophore mutants were homologous with B. pertussis chromosomal DNA, while bioassays suggested siderophore cross-feeding between B. pertussis and B. bronchiseptica. These results indicate probable similarity between the siderophore biosynthetic and transport systems of the two species. Originally published Journal of Bacteriology, Vol. 175, No. 4, Feb 1993
    URI
    http://hdl.handle.net/10342/3437
    Subject
     Bordetella bronchiseptica; Siderophore activity; Iron acquisition 
    Date
    1993-02
    Citation:
    APA:
    Armstrong, Sandra K., & Clements, Mark O.. (February 1993). Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.. Journal of Bacteriology, 175(4), 1144- 1152. Retrieved from http://hdl.handle.net/10342/3437

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Armstrong, Sandra K., and Clements, Mark O.. "Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.". Journal of Bacteriology. 175:4. (1144-1152), February 1993. December 01, 2023. http://hdl.handle.net/10342/3437.
    Chicago:
    Armstrong, Sandra K. and Clements, Mark O., "Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.," Journal of Bacteriology 175, no. 4 (February 1993), http://hdl.handle.net/10342/3437 (accessed December 01, 2023).
    AMA:
    Armstrong, Sandra K., Clements, Mark O.. Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.. Journal of Bacteriology. February 1993; 175(4): 1144-1152. http://hdl.handle.net/10342/3437. Accessed December 01, 2023.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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