• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • ECU Main Campus
    • College of Health and Human Performance
    • Kinesiology
    • View Item
    •   ScholarShip Home
    • ECU Main Campus
    • College of Health and Human Performance
    • Kinesiology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Cleaved Caspase-3 Response to Acute Resistance Exercise in Young and Old Men and Women : Relationship to Muscle Glycogen Content and 5'-AMP-Activated Protein Kinase (AMPK) Activity

    Thumbnail
    View/ Open
    Choplin_ecu_0600M_10448.pdf (1.156Mb)

    Show full item record
    Author
    Choplin, Eric Seneca
    Abstract
    In addition to suppressing protein synthesis and activating protein degradation in skeletal muscle and other cell types, 5'-AMP-activated protein kinase (AMPK) is known to stimulate nuclear apoptosis in non-muscle cells through cleaved (activated) caspase-3, one of the final steps in the apoptosis cascade. Although it is unknown whether AMPK stimulates caspase-3 cleavage or nuclear apoptosis in skeletal muscle cells in vivo, AMPK activity and nuclear apoptosis are elevated at rest in aged rat skeletal muscle. AMPK phosphorylation and activity are also higher in old vs. young rats and humans in response to overload or resistance exercise. Furthermore, older individuals display lower muscle glycogen content, a condition known to accentuate AMPK activity at rest and during aerobic exercise. We hypothesized that skeletal muscle cleaved caspase-3 content would be higher after acute resistance exercise in older versus younger individuals. Seven young (21.7 ± 2.1 yrs) and 11 old (67.0 ± 8.6 yrs) subjects performed an acute bout of leg extension resistance exercise. Muscle biopsies were obtained pre-exercise (PRE), immediately post-exercise (0P), 1-hour post-exercise (1P), and 2-hours post-exercise (2P). Glycogen content was measured in muscle samples, as were the phosphorylations (via western blot) of AMPK and acetyl-CoA carboxylase (ACC; a marker of AMPK activity). Procaspase-3 and cleaved (activated) caspase-3 contents were also assessed via western blot. AMPK phosphorylation was significantly (p < 0.05) increased in old, but not young, subjects immediately post-exercise. In both age groups, AMPK activity (assessed by ACC phosphorylation) was elevated vs. PRE at the 0P and 1P time points, and cleaved caspase-3 content was elevated vs. PRE at the 0P, 1P, and 2P time points. However, there was no effect of exercise on procaspase-3 content in either age group, and no differences between age groups in AMPK activity, procaspase-3 content, or cleaved caspase-3 content at any time point. There were significant, or close to significant, relationships between glycogen content and AMPK activity at time points PRE, 0P, and 1P regardless of age. However, no significant correlations between AMPK activity and cleaved caspase-3 content were observed at any time point. In summary, these data indicate that cleaved caspase-3 (e.g., caspase-3 activity) increases in response to acute resistance exercise in both young and old subjects. However, exercise-induced AMPK activation may not be the mechanism by which this occurs.  
    URI
    http://hdl.handle.net/10342/3652
    Subject
     Physiology; Aging; AMPK; Sarcopenia; Skeletal muscle; Biology, Physiology 
    Date
    2011
    Citation:
    APA:
    Choplin, Eric Seneca. (January 2011). Cleaved Caspase-3 Response to Acute Resistance Exercise in Young and Old Men and Women : Relationship to Muscle Glycogen Content and 5'-AMP-Activated Protein Kinase (AMPK) Activity (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/3652.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Choplin, Eric Seneca. Cleaved Caspase-3 Response to Acute Resistance Exercise in Young and Old Men and Women : Relationship to Muscle Glycogen Content and 5'-AMP-Activated Protein Kinase (AMPK) Activity. Master's Thesis. East Carolina University, January 2011. The Scholarship. http://hdl.handle.net/10342/3652. September 25, 2023.
    Chicago:
    Choplin, Eric Seneca, “Cleaved Caspase-3 Response to Acute Resistance Exercise in Young and Old Men and Women : Relationship to Muscle Glycogen Content and 5'-AMP-Activated Protein Kinase (AMPK) Activity” (Master's Thesis., East Carolina University, January 2011).
    AMA:
    Choplin, Eric Seneca. Cleaved Caspase-3 Response to Acute Resistance Exercise in Young and Old Men and Women : Relationship to Muscle Glycogen Content and 5'-AMP-Activated Protein Kinase (AMPK) Activity [Master's Thesis]. Greenville, NC: East Carolina University; January 2011.
    Collections
    • Kinesiology
    • Master's Theses
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback