• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Academic Affairs
    • College of Health and Human Performance
    • Kinesiology
    • View Item
    •   ScholarShip Home
    • Academic Affairs
    • College of Health and Human Performance
    • Kinesiology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    THE EFFECTS OF CIRCADIAN RHYTHM ON INFLAMMATORY RESPONSE AND CYTOKINE LEVELS IN HEPATIC MACROPHAGES

    Thumbnail
    View/ Open
    Orcino_ecu_0600O_11316.pdf (1.212Mb)
    thesissignatures.pdf (445.5Kb)

    Show full item record
    Author
    Orcino, Christina Michelle
    Abstract
    In mammals, many aspects of daily behavior and physiology such as the sleep-wake cycle, body temperature, and liver metabolism are regulated by endogenous circadian mechanisms. Despite tissue-specific physiological differences, these circadian rhythms share a highly conserved negative feedback mechanism, consisting of transcriptional activators and repressors. This feedback loop system is critical for normal physiology and behavior, and its disruption can lead to sleep disorders, metabolic syndrome, cancer, and a whole host of other diseases, including immune related dysfunction. In this study, the loss of Bmal, a key transcriptional activator, and its ability to regulate a macrophage response was evaluated. To do this, a loxP-cre system was used to create mice with a macrophage-specific deletion of Bmal (lysZ-Bmal -/-). This was the first look at the role of macrophage-specific circadian gene expression in regulation of hepatic immune response and its influence on macrophage differentiation and inflammatory liver disease in vivo. Cre-negative littermate controls and lysZ-Bmal -/- mice were stimulated with the endotoxin lipopolysaccharide (LPS) to provoke a pro-inflammatory response. In lysZ-Bmal -/- mice, cells stimulated with LPS had an exacerbated pro-inflammatory response but a blunted anti-inflammatory response compared to cells from cre-negative littermates. LysZ-Bmal -/- mice also had larger liver to bodyweight ratios as well as increased relative mRNA levels of critical pro-inflammatory cytokines and serum ALT. In summary, this study suggests that circadian regulation in macrophages may be important for lipid control and that Bmal regulates a pro-inflammatory response in hepatic macrophages.  
    URI
    http://hdl.handle.net/10342/4696
    Subject
     Genetics; Biochemistry; Health sciences 
    Date
    2014
    Citation:
    APA:
    Orcino, Christina Michelle. (January 2014). THE EFFECTS OF CIRCADIAN RHYTHM ON INFLAMMATORY RESPONSE AND CYTOKINE LEVELS IN HEPATIC MACROPHAGES (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/4696.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Orcino, Christina Michelle. THE EFFECTS OF CIRCADIAN RHYTHM ON INFLAMMATORY RESPONSE AND CYTOKINE LEVELS IN HEPATIC MACROPHAGES. Master's Thesis. East Carolina University, January 2014. The Scholarship. http://hdl.handle.net/10342/4696. August 17, 2022.
    Chicago:
    Orcino, Christina Michelle, “THE EFFECTS OF CIRCADIAN RHYTHM ON INFLAMMATORY RESPONSE AND CYTOKINE LEVELS IN HEPATIC MACROPHAGES” (Master's Thesis., East Carolina University, January 2014).
    AMA:
    Orcino, Christina Michelle. THE EFFECTS OF CIRCADIAN RHYTHM ON INFLAMMATORY RESPONSE AND CYTOKINE LEVELS IN HEPATIC MACROPHAGES [Master's Thesis]. Greenville, NC: East Carolina University; January 2014.
    Collections
    • Kinesiology
    • Master's Theses
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback