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    Analysis of the Maize dicer-like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence

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    Author
    Field, Sterling; Thompson, Beth
    Abstract
    Sexual reproduction in plants requires development of haploid gametophytes from somatic tissues. Pollen is the male gametophyte and develops within the stamen; defects in the somatic tissues of the stamen and in the male gametophyte itself can result in male sterility. The maize fuzzy tassel (fzt) mutant has a mutation in dicer-like1 (dcl1), which encodes a key enzyme required for microRNA (miRNA) biogenesis. Many miRNAs are reduced in fzt, and fzt mutants exhibit a broad range of developmental defects, including male sterility. To gain further insight into the roles of miRNAs in maize stamen development, we conducted a detailed analysis of the male sterility defects in fzt mutants. Early development was normal in fzt mutant anthers, however fzt anthers arrested in late stages of anther maturation and did not dehisce. A minority of locules in fzt anthers also exhibited anther wall defects. At maturity, very little pollen in fzt anthers was viable or able to germinate. Normal pollen is tricellular at maturity; pollen from fzt anthers included a mixture of unicellular, bicellular, and tricellular pollen. Pollen from normal anthers is loaded with starch before dehiscence, however pollen from fzt anthers failed to accumulate starch. Our results indicate an absolute requirement for miRNAs in the final stages of anther and pollen maturation in maize. Anther wall defects also suggest that miRNAs have key roles earlier in anther development. We discuss candidate miRNAs and pathways that might underlie fzt anther defects, and also note that male sterility in fzt resembles water deficit-induced male sterility, highlighting a possible link between development and stress responses in plants.
    URI
    http://hdl.handle.net/10342/5192
    Subject
     Pollen; Stamens; MicroRNAs; Anthers; Maize; Cellular stress responses; Flowering plants 
    Date
    2016-01-08
    Citation:
    APA:
    Field, Sterling, & Thompson, Beth. (January 2016). Analysis of the Maize dicer-like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence. , (), - like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence. PLoS ONE 11(1): e0146534. doi:10.1371/journal.pone.0146534. Retrieved from http://hdl.handle.net/10342/5192

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Field, Sterling, and Thompson, Beth. "Analysis of the Maize dicer-like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence". . . (), January 2016. September 22, 2023. http://hdl.handle.net/10342/5192.
    Chicago:
    Field, Sterling and Thompson, Beth, "Analysis of the Maize dicer-like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence," , no. (January 2016), http://hdl.handle.net/10342/5192 (accessed September 22, 2023).
    AMA:
    Field, Sterling, Thompson, Beth. Analysis of the Maize dicer-like1 Mutant, fuzzy tassel, Implicates MicroRNAs in Anther Maturation and Dehiscence. . January 2016; (): . http://hdl.handle.net/10342/5192. Accessed September 22, 2023.
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    • 2015-2016 Open Access Publishing Fund
    • Biology
    • Open Access Fund
    • Open Access Publishing Support Fund

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