Browsing Biology by Author "Christensen, Tim"
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Characterization of the GINS subunit psf1 in Drosophila Melanogaster
Sufrinko, Brian (East Carolina University, 2010)GINS (Go Ichi Ni San; 5,1,2,3 in Japanese) is a heterotetrameric protein complex known to be essential for the establishment of DNA replication forks and the progression of the replisome. The GINS complex is known to be ... -
Characterization of the Role of Mcm10 in DNA Replication in Drosophila melanogaster
Dalia, Ritu (East Carolina University, 2013)Replication of the genome and proper formation, and packaging, of chromatin are processes essential to eukaryotic life. Maintenance of epigenetic chromatin states is essential for faithfully reproducing the transcriptional ... -
Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster
Hunter, Chad Michael (East Carolina University, 2011)DNA is the genetic material for all living organisms which is constantly being unpackaged, replicated and repackaged. The replication of this genetic material involves numerous different proteins; however, DNA polymerase ... -
Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression
Gosnell, Justin A.; Christensen, Tim (2011)Background Proper coordination of the functions at the DNA replication fork is vital to the normal functioning of a cell. Specifically the precise coordination of helicase and polymerase activity is crucial for efficient ... -
Drosophila Ctf4 is essential for genome stability and normal cell cycle progression
Gosnell, Justin A. (East Carolina University, 2010)Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding ... -
Drosophila Psf2 Has a Role In Chromosome Condensation
Chmielewski, Jeffrey Patrick (East Carolina University, 2012)The condensation state of chromosomes is a critical parameter in multiple processes within the cell. Failures in the maintenance of appropriate condensation states may lead to genomic instability, mis-expression of genes, ... -
Elucidating Gene Function with the use of Genome Wide Association Study (GWAS)
Ransdell, Garrett (East Carolina University, 2015)DNA replication and cell proliferation are intensely studied subjects. There have been countless experiments and hours put into better understanding how DNA is replicated and compacted, and how cells are able to proliferate ... -
ESTABLISHING LINKAGE BETWEEN GINS COMPLEX SUB-UNIT Sld5 AND CHECKPOINT PROTEIN Chk2 (loki) USING DROSOPHILA MELANOGASTER AS MODEL ORGANISM
Devadasan, Divya (East Carolina University, 2012)Eukaryotic DNA replication is controlled by a number of proteins that ensures the process takes place accurately. GINS, a hetero-tetrameric protein complex is known to be essential for the initiation and progression of ... -
Evaluating The Essential Functions Of The Replication Factor Mcm10 In Drosophila Melanogaster
Reubens, Michael C. (East Carolina University, 2015)Often referred to as miraculous, life as we know depends not upon miracles but on a series of highly orchestrated and regulated biochemical processes collectively known as the cell cycle. It is through these series of ... -
A Genetic Screen for Mcm10: Interactions Between Mcm10 and Deficiency Regions on the Third Chromosome of Drosophila Melanogaster
Anstead, Bryan (2015)Mis-regulation of the Mcm10 gene has been linked with multiple forms of cancer, including many forms of breast cancer (Thu, Y. 2014). Despite this, the role of Mcm10 in cancer is not yet fully known. One way to study a ... -
Interaction and Functional Analysis of Drosophila Mcm10
Apger, Jennifer (East Carolina University, 2010)In eukaryotic cells, DNA replication and the subsequent packaging of DNA into specific chromatin states are essential processes for the transmission of genetic material and genomic stability which may be linked by interactions ... -
Investigating the genetic interaction between DNA replication proteins Mcm10 and RecQ4 in Drosophila melanogaster
Knuckles, Christopher I (East Carolina University, 2017-12-12)Necessary to the survival of cellular life is proper replication and maintenance of the genome. Replication proteins Mcm10 and RecQ4 have well-characterized essential roles in assembly, initiation, proper functioning of ... -
Investigating the Interaction of RecQL4 and Mcm10 in Drosophila melanogaster
Rummings, Wayne Anthony, Jr. (East Carolina University, 2014)Accurate duplication and regulation of the eukaryotic genome requires precise coordination among multiple replication factors. RecQL4, the least characterized RecQ protein, is a 1208 amino acid protein containing a centrally ... -
MCM10 Genetic Screen Using Drosophila melanogaster
Dinter, Heinz P (East Carolina University, 2016-04-28)Genes and the proteins they encode are responsible for virtually every aspect of life. The vast array of biological processes make it difficult to identify genes to study that are relevant to human diseases. MCM10 (short ... -
Psf2, a member of the heterotetrameric GINS Complex, plays a role in cell cycle progression and maintenance of genomic integrity
Henderson, Laura M. (East Carolina University, 2010)Multiple proteins are involved in the complete and accurate replication of the genome during S phase of the cell cycle. At the G1/S phase transition, the heterotetrameric GINS complex is recruited to the origin, and ... -
Sld5, A Subunit of the Heterotetrameric GINS Complex is Necessary for Normal Cell Cycle Progression and Genomic Stability
Gouge, Catherine Anne (East Carolina University, 2010)Sld5 is one component of the GINS heterotetrameric complex essential to DNA replication. Specifically, GINS is known for its integral role during the G1 to S phase transition in the cell cycle. The GINS complex is comprised ...