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    Label-free classification of cultured cells through diffraction imaging

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    Author
    Dong, Ke; Feng, Yuanming; Jacobs, Kenneth M.; Lu, Jun Q.; Brock, R. Scott; Yang, Li V.; Bertrand, Fred E.; Farwell, Mary A.; Hu, Xin-Hua
    Abstract
    Automated classification of biological cells according to their 3D morphology is highly desired in a flow cytometer setting. We have investigated this possibility experimentally and numerically using a diffraction imaging approach. A fast image analysis software based on the gray level co-occurrence matrix (GLCM) algorithm has been developed to extract feature parameters from measured diffraction images. The results of GLCM analysis and subsequent classification demonstrate the potential for rapid classification among six types of cultured cells. Combined with numerical results we show that the method of diffraction imaging flow cytometry has the capacity as a platform for high-throughput and label-free classification of biological cells.
    URI
    http://hdl.handle.net/10342/5774
    Date
    2011-05
    Citation:
    APA:
    Dong, Ke, & Feng, Yuanming, & Jacobs, Kenneth M., & Lu, Jun Q., & Brock, R. Scott, & Yang, Li V., & Bertrand, Fred E., & Farwell, Mary A., & Hu, Xin-Hua. (May 2011). Label-free classification of cultured cells through diffraction imaging. Biomedical Optics Express, (2:6), p.1717-1726. Retrieved from http://hdl.handle.net/10342/5774

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Dong, Ke, and Feng, Yuanming, and Jacobs, Kenneth M., and Lu, Jun Q., and Brock, R. Scott, and Yang, Li V., and Bertrand, Fred E., and Farwell, Mary A., and Hu, Xin-Hua. "Label-free classification of cultured cells through diffraction imaging". Biomedical Optics Express. 2:6. (1717-1726.), May 2011. February 24, 2021. http://hdl.handle.net/10342/5774.
    Chicago:
    Dong, Ke and Feng, Yuanming and Jacobs, Kenneth M. and Lu, Jun Q. and Brock, R. Scott and Yang, Li V. and Bertrand, Fred E. and Farwell, Mary A. and Hu, Xin-Hua, "Label-free classification of cultured cells through diffraction imaging," Biomedical Optics Express 2, no. 6 (May 2011), http://hdl.handle.net/10342/5774 (accessed February 24, 2021).
    AMA:
    Dong, Ke, Feng, Yuanming, Jacobs, Kenneth M., Lu, Jun Q., Brock, R. Scott, Yang, Li V., Bertrand, Fred E., Farwell, Mary A., Hu, Xin-Hua. Label-free classification of cultured cells through diffraction imaging. Biomedical Optics Express. May 2011; 2(6) 1717-1726. http://hdl.handle.net/10342/5774. Accessed February 24, 2021.
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