Browsing Chemistry by Title
Now showing items 51-70 of 120
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Identification of the Cu(II) Coordinating Residues in the Prion Protein by Metal-Catalyzed Oxidation Mass Spectrometry: Evidence for Multiple Isomers at Low Cu(II) Loadings
(East Carolina University, 2008-09-02)While the Cu(II) binding sites of the prion protein have been well studied under Cu-saturation conditions, the identity of the residues involved in coordinating Cu(II) at low stoichiometries and the order in which the ... -
Identifying compensatory genomic changes due to nickel (Ni2+) exposure: electrochemistry and quantifying DNA damage
(East Carolina University, 2020-11-24)Nickel is a toxic heavy metal that has been shown to cause adverse healthconditions in organisms. DNA damage has been observed in nematodes in response to nickel exposure. Nematodes originating from environments with high ... -
The implementation of a GC/MS data system using DISNET: a Distributed Instrument System NETwork
(East Carolina University, 1984)The primary goal of a computer network is to achieve resource sharing between several computers. Resource sharing is most easily performed at the application program level, with lower layers of software providing communications ... -
The implementation of a GC/MS data system using DISNET: a Distributed Instrument System NETwork
(East Carolina University, 1984) -
Improving Prostamide Anti-Cancer Activity Through Derivatization and Micellar Delivery
(East Carolina University, 2023-04-28)Colorectal cancer is the fourth most common cancer diagnosis per year as well as the fourth highest rate of death per year according to the Centers for Disease Control. Approximately 1/3rd of the diagnosed colorectal cancer ... -
Improving the Reliability of Peptide-Zinc (II) Binding Constant Determination with Mass Spectrometry
(East Carolina University, 2012)Electrospray Ionization Mass Spectrometry (ESI-MS) has become a powerful technique for analyzing protein- and peptide-ligand interactions and quantifying their binding constants (K[subscript]b). However, ESI-MS as an ... -
Insight Into the Location of DNA Xenobiotic Damage by Mass Spectrometry
(2014)Damage to DNA by a bioactivated xenobiotic typically occurs at specific sites within the genome, called hotspots. An example of this is benzo[a]-pyrene (BP), a xenobiotic that enters the body via cigarette smoking. ... -
Installation of Metal-Binding Functionality at Lysine Residues via Azide-Alkyne "Click Chemistry"
(2015-04-29)A novel non-natural amino acid designed to bind divalent metal ions, dubbed Lys(PYRIT), was prepared in a “click reaction” by treating Fmoc-Lys(N3)-OH with 2-ethynylpyridine and Cu(I). It was incorporated into a 12-residue ... -
Investigating Intra- and Inter-Molecular Coevolution of Intrinsically Disordered Protein, Prothymosin-alpha
(East Carolina University, 1/13/16)Prothymosin-α (ProTα) is a small, highly acidic protein found in the nuclei of virtually all mammalian tissues. It belongs to a class of proteins known for their lack of a rigid three-dimensional structure called intrinsically ... -
Investigation Into Intermolecular Interactions of Gd3N@C80(OH)n Using Fluorescence Spectroscopy
(2014)The aim of this experiment was to investigate the intermolecular interactions of the endohedral metallofulerene complex in water and various metal cation solutions. These interactions were observed using a methodology ... -
Investigation Into The Scope, Limitations, And Mechanistic Aspects Of A Regioselective Acylation Of Cycloplatinated Complexes
(East Carolina University, 2015)In the reaction of N-isopropyl-N-phenyl-2,2'-bipyridin-6-amine with potassium tetrachloroplatinate, the selective C-H bond activation was complicated by the low selectivity of sp² C-H bond activation in acetonitrile and ... -
Investigation of Molecular Mechanism of Transthyretin Oligomerization Associated with ATTR Amyloidosis
(East Carolina University, 2022-04-27)Transthyretin amyloidosis (ATTR) has classically been diagnosed predominantly post-observation of amyloid fibril deposition on soft tissues within the body, however, recent studies have shown that oligomers which arise ... -
Investigation of possible driving forces on counter-intuitive thermal collapse of intrinsically disordered proteins
(East Carolina University, 2016-08-18)Intrinsically disordered proteins are an emerging class of proteins that contain no secondary or tertiary structure. Through bioinformatics studies it has been predicted that a third of the human proteome consists of ... -
Investigation of the Zinc binding region of Prothymosin-alpha : A spectroscopic and thermodynamic approach to study metal binding in Intrinsically disordered proteins
(East Carolina University, 2014)The goal of this study is to provide a deeper insight into how metals bind intrinsically disordered proteins (IDPs) by taking zinc binding to prothymosin-alpha as a case study. The involvement of metals in several diseases ... -
Kinetic Modeling of Batch Reaction Processes
(East Carolina University, 2012)Kinetic models have been demonstrated to be useful in on-line batch monitoring systems. The ability to monitor a reaction in real time is invaluable to the production process of industrial and pharmaceutical products. ... -
KINETIC MODELING OF DISSOLUTION AND CRYSTALLIZATION OF BATCH REACTIONS WITH IN SITU SPECTROSCOPIC MEASUREMENTS
(East Carolina University, 2012)The use of Process Analytical Technology (PAT) recommended by the Food and Drug Administration (FDA) has significantly increased during the past years in the design, control and monitoring of pharmaceutical or chemical ... -
Membrane Localization of Pyrene-Trehalose Conjugates (PYRETs)
(East Carolina University, 2018-05-01)The disaccharide trehalose is found in many organisms that can survive in extremely cold or dry environments. According to the “hydration hypothesis,” trehalose interacts with the glycerol/phosphate regions of lipid bilayers ... -
Method Development and Validation for the Rapid Detection of Organophosphates in Blood Plasma from Obese, Obese-Diabetic, and Lean Patients from Eastern North Carolina
(East Carolina University, 2017-07-13)The incidence of diabetes and rate of obesity is on the rise, along with the use of organophosphorus insecticides in the United States. Organophosphates (OP), a specific class of pesticide that is biodegradable and readily ... -
Modern and Classic Quantum Chemistry: Modeling Rhodium-Catalyzed Hydroacylation with Advanced Tools and the Development and Application of Software to Examine Simple Bonding Interactions in a Basis of Molecular Fragments
(East Carolina University, 8/5/2020)In this dissertation, both modern and classic computational methods are utilized to examine two problems of interest in the field of chemistry. The first problem involves a complex rhodium-catalyzed hydroacylation reaction ...