Structural insights into flagellar stator– rotor interactions
Author
Chang, Yunjie; Moon, Ki Hwan; Zhao, Xiaowei; Norris, Steven J; Motaleb, MD A; Liu, Jun
Abstract
The bacterial flagellar motor is a molecular machine that can rotate the flagellar
filament at high speed. The rotation is generated by the stator–rotor interaction, coupled with an
ion flux through the torque-generating stator. Here we employed cryo-electron tomography to
visualize the intact flagellar motor in the Lyme disease spirochete, Borrelia burgdorferi. By
analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the
stator complex in situ, but also revealed the stator–rotor interaction at an unprecedented detail.
Importantly, the stator–rotor interaction induces a conformational change in the flagella C-ring.
Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate
the similar conformational change, we propose that the proton-driven torque is responsible for the
conformational change required for flagellar rotation.
Date
2019-07-17
Citation:
APA:
Chang, Yunjie, & Moon, Ki Hwan, & Zhao, Xiaowei, & Norris, Steven J, & Motaleb, MD A, & Liu, Jun. (July 2019).
Structural insights into flagellar stator– rotor interactions.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8326
MLA:
Chang, Yunjie, and Moon, Ki Hwan, and Zhao, Xiaowei, and Norris, Steven J, and Motaleb, MD A, and Liu, Jun.
"Structural insights into flagellar stator– rotor interactions". .
. (),
July 2019.
May 08, 2024.
http://hdl.handle.net/10342/8326.
Chicago:
Chang, Yunjie and Moon, Ki Hwan and Zhao, Xiaowei and Norris, Steven J and Motaleb, MD A and Liu, Jun,
"Structural insights into flagellar stator– rotor interactions," , no.
(July 2019),
http://hdl.handle.net/10342/8326 (accessed
May 08, 2024).
AMA:
Chang, Yunjie, Moon, Ki Hwan, Zhao, Xiaowei, Norris, Steven J, Motaleb, MD A, Liu, Jun.
Structural insights into flagellar stator– rotor interactions. .
July 2019;
():
.
http://hdl.handle.net/10342/8326. Accessed
May 08, 2024.
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