Structural insights into flagellar stator– rotor interactions
Chang, Yunjie; Moon, Ki Hwan; Zhao, Xiaowei; Norris, Steven J; Motaleb, MD A; Liu, Jun
The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator–rotor interaction, coupled with an ion flux through the torque-generating stator. Here we employed cryo-electron tomography to visualize the intact flagellar motor in the Lyme disease spirochete, Borrelia burgdorferi. By analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the stator complex in situ, but also revealed the stator–rotor interaction at an unprecedented detail. Importantly, the stator–rotor interaction induces a conformational change in the flagella C-ring. Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate the similar conformational change, we propose that the proton-driven torque is responsible for the conformational change required for flagellar rotation.
Chang, Yunjie, & Moon, Ki Hwan, & Zhao, Xiaowei, & Norris, Steven J, & Motaleb, MD A, & Liu, Jun. (July 2019). Structural insights into flagellar stator– rotor interactions. eLife, (e48979. Retrieved from http://hdl.handle.net/10342/8326
Chang, Yunjie, and Moon, Ki Hwan, and Zhao, Xiaowei, and Norris, Steven J, and Motaleb, MD A, and Liu, Jun. "Structural insights into flagellar stator– rotor interactions". eLife. . (e48979.), July 2019. July 29, 2021. http://hdl.handle.net/10342/8326.
Chang, Yunjie and Moon, Ki Hwan and Zhao, Xiaowei and Norris, Steven J and Motaleb, MD A and Liu, Jun, "Structural insights into flagellar stator– rotor interactions," eLife 8, no. (July 2019), http://hdl.handle.net/10342/8326 (accessed July 29, 2021).
Chang, Yunjie, Moon, Ki Hwan, Zhao, Xiaowei, Norris, Steven J, Motaleb, MD A, Liu, Jun. Structural insights into flagellar stator– rotor interactions. eLife. July 2019; 8() e48979. http://hdl.handle.net/10342/8326. Accessed July 29, 2021.