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    SYNTHESIS AND ANALYSIS OF FLUORESCENT ANTIFREEZE GLYCOPROTEIN MIMIC PEPTIDES FOR DETERMINATION OF THE ICE BINDING MECHANISM

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    KOUFAS-MASTERSTHESIS-2021.pdf (37.75Mb)

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    Author
    Koufas, Megan Gabriele
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    This item will be available on: 2023-12-01
    Abstract
    Properties of antifreeze glycoproteins (AFGPs) are well known; however, it is unclear which molecular components of the proteins are directly responsible for binding to the ice surface. Previous molecular dynamics studies have implicated hydrophobic methyl groups in ice binding, while microscopy experiments have implicated polar hydroxyl groups from the galactosamine units. This thesis describes the synthesis of non-glycosylated AFGP mimic sequences which have been labeled with a water-sensitive naphthalimide fluorophore at several locations. In principle, the emission intensity of these systems should change if ice binding removes the fluorophore from contact with liquid water. Circular dichroism analysis suggests that the presence of the fluorescent label at the N-terminal amino acid induces polyproline II structure to the peptide. C-terminal labeling does not induce the same structuring. Results from fluorescence spectroscopy are consistent with the formation of microscopic ice crystals at or below 0°C, which serve to draw the peptides out of the sample beam and thereby decrease the fluorescence intensity. To our knowledge, this is the first example of bulk solution fluorescence being used to demonstrate ice binding through hydrophobic groups.
    URI
    http://hdl.handle.net/10342/9759
    Subject
     Antifreeze Glycoprotein; AFGP; Fluorescence 
    Date
    2021-09-13
    Citation:
    APA:
    Koufas, Megan Gabriele. (September 2021). SYNTHESIS AND ANALYSIS OF FLUORESCENT ANTIFREEZE GLYCOPROTEIN MIMIC PEPTIDES FOR DETERMINATION OF THE ICE BINDING MECHANISM (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/9759.)

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    MLA:
    Koufas, Megan Gabriele. SYNTHESIS AND ANALYSIS OF FLUORESCENT ANTIFREEZE GLYCOPROTEIN MIMIC PEPTIDES FOR DETERMINATION OF THE ICE BINDING MECHANISM. Master's Thesis. East Carolina University, September 2021. The Scholarship. http://hdl.handle.net/10342/9759. May 17, 2022.
    Chicago:
    Koufas, Megan Gabriele, “SYNTHESIS AND ANALYSIS OF FLUORESCENT ANTIFREEZE GLYCOPROTEIN MIMIC PEPTIDES FOR DETERMINATION OF THE ICE BINDING MECHANISM” (Master's Thesis., East Carolina University, September 2021).
    AMA:
    Koufas, Megan Gabriele. SYNTHESIS AND ANALYSIS OF FLUORESCENT ANTIFREEZE GLYCOPROTEIN MIMIC PEPTIDES FOR DETERMINATION OF THE ICE BINDING MECHANISM [Master's Thesis]. Greenville, NC: East Carolina University; September 2021.
    Collections
    • Master's Theses
    Publisher
    East Carolina University

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