Anatomical-Molecular Distribution of EphrinA1 in Infarcted Mouse Heart Using MALDI Mass Spectrometry Imaging

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dc.contributor.authorLefcoski, Stephan
dc.contributor.authorKew, Kimberly
dc.contributor.authorReece, Shaun
dc.contributor.authorTorres, Maria J.
dc.contributor.authorParks, Justin C.
dc.contributor.authorde Castro Brás, Lisandra E.
dc.contributor.authorVirag, Jitka A. I.
dc.date.accessioned2019-03-14T20:42:39Z
dc.date.available2019-03-14T20:42:39Z
dc.date.issued2018-01-05
dc.description.abstractEphrinA1 is a tyrosine kinase receptor localized in the cellular membrane of healthy cardiomyocytes, the expression of which is lost upon myocardial infarction (MI). Intra-cardiac injection of the recombinant form of ephrinA1 (ephrinA1-Fc) at the time of ligation in mice has shown beneficial effects by reducing infarct size and myocardial necrosis post-MI. To date, immunohistochemistry and Western blotting comprise the only experimental approaches utilized to localize and quantify relative changes of ephrinA1 in sections and homogenates of whole left ventricle, respectively. Herein, we used matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) coupled with a time-of-flight mass spectrometer (MALDI/TOF MS) to identify intact as well as tryptic fragments of ephrinA1 in healthy controls and acutely infarcted murine hearts. The purpose of the present study was 3-fold: (1) to spatially resolve the molecular distribution of endogenous ephrinA1, (2) to determine the anatomical expression profile of endogenous ephrinA1 after acute MI, and (3) to identify molecular targets of ephrinA1-Fc action post-MI. The tryptic fragments detected were identified as the ephrinA1-isoform with 38% and 34% sequence coverage and Mascot scores of 25 for the control and MI hearts, respectively. By using MALDI-MSI, we have been able to simultaneously measure the distribution and spatial localization of ephrinA1, as well as additional cardiac proteins, thus offering valuable information for the elucidation of molecular partners, mediators, and targets of ephrinA1 action in cardiac muscle.en_US
dc.description.sponsorshipOpen Access Funden_US
dc.identifier.citationLefcoski, S., Kew, K., Reece, S. et al. J. Am. Soc. Mass Spectrom. (2018) 29: 527. https://doi.org/10.1007/s13361-017-1869-7en_US
dc.identifier.doi10.1007/s13361-017-1869-7
dc.identifier.pmid29305797en_US
dc.identifier.urihttp://hdl.handle.net/10342/7103
dc.language.isoen_USen_US
dc.relation.urihttps://link.springer.com/article/10.1007%2Fs13361-017-1869-7#citeasen_US
dc.subjectMALDI/IMSen_US
dc.subjectEphrinA1en_US
dc.subjectMyocardial infarctionen_US
dc.titleAnatomical-Molecular Distribution of EphrinA1 in Infarcted Mouse Heart Using MALDI Mass Spectrometry Imagingen_US
dc.typeArticleen_US
ecu.journal.issue3en_US
ecu.journal.nameJournal of The American Society for Mass Spectrometryen_US
ecu.journal.pages527-534en_US
ecu.journal.volume29en_US

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