EXPRESSION AND PURIFICATION OF THE REGULATORY DOMAIN OF HUMAN CARDIAC TROPONIN C (HCTNC): A PROTEIN METHOD OPTIMIZATION STUDY

Abstract

The purpose of this experimentation was to optimize the purification methodology for Human Cardiac Troponin C (HcTnC). This was done by replacing the old methodology of the size exclusion column and ion exchange column, with a phenyl sepharose column. This increased the protein concentration from 0.095 mg/mL to 0.251 mg/mL. This is important because it allows HcTnC to be purified faster with a higher yield, so more experimentation can be conducted. Further experimentation should be done to optimize the elution gradient as well as optimize the protein induction.