EXPRESSION AND PURIFICATION OF THE REGULATORY DOMAIN OF HUMAN CARDIAC TROPONIN C (HCTNC): A PROTEIN METHOD OPTIMIZATION STUDY

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dc.access.optionOpen Access
dc.contributor.advisorAnderson, Eric
dc.contributor.advisorSpuches , Anne
dc.contributor.authorOliveira, Luis Felipe
dc.contributor.departmentBiology
dc.date.accessioned2023-07-13T16:48:47Z
dc.date.available2023-07-13T16:48:47Z
dc.date.created2023-05
dc.date.issued2023-04-26
dc.date.submittedMay 2023
dc.date.updated2023-06-30T13:45:09Z
dc.degree.departmentBiology
dc.degree.disciplineBiology
dc.degree.grantorEast Carolina University
dc.degree.levelUndergraduate
dc.degree.nameBS
dc.description.abstractThe purpose of this experimentation was to optimize the purification methodology for Human Cardiac Troponin C (HcTnC). This was done by replacing the old methodology of the size exclusion column and ion exchange column, with a phenyl sepharose column. This increased the protein concentration from 0.095 mg/mL to 0.251 mg/mL. This is important because it allows HcTnC to be purified faster with a higher yield, so more experimentation can be conducted. Further experimentation should be done to optimize the elution gradient as well as optimize the protein induction.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10342/12966
dc.publisherEast Carolina University
dc.subjectChromatography, Biology, Biochemistry, Protein, Purification, FPLC,
dc.titleEXPRESSION AND PURIFICATION OF THE REGULATORY DOMAIN OF HUMAN CARDIAC TROPONIN C (HCTNC): A PROTEIN METHOD OPTIMIZATION STUDY
dc.typeHonors Thesis
dc.type.materialtext

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