ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA

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dc.access.optionOpen Access
dc.contributor.advisorGeyer, Christopher B.
dc.contributor.authorKirsanov, Oleksandr
dc.contributor.departmentAnatomy and Cell Biology
dc.date.accessioned2018-08-14T15:15:09Z
dc.date.available2020-08-01T08:01:53Z
dc.date.created2018-08
dc.date.issued2018-07-24
dc.date.submittedAugust 2018
dc.date.updated2018-08-09T20:04:33Z
dc.degree.departmentAnatomy and Cell Biology
dc.degree.disciplineMS-Biomedical Science
dc.degree.grantorEast Carolina University
dc.degree.levelMasters
dc.degree.nameM.S.
dc.description.abstractSpermatogenesis in the mammalian testis results in the daily production of millions of spermatozoa. This developmental process is founded upon the actions of a small population of spermatogonial stem cells (SSCs). As SSCs divide, their progeny must either remain an SSC to maintain the stem cell pool (self-renewal) or become an undifferentiated progenitor spermatogonium that proliferates prior to differentiating in response to retinoic acid (RA) and entering meiosis. Our laboratory is focused on understanding the mechanisms that regulate these fate decisions as well as defining the changes that occur downstream of RA that prepare spermatogonia for entry into meiosis. Currently, researchers have been unable to readily isolate pure populations of spermatogonia in the developing or adult testis. To address this, I have examined multiple transgenic mouse models to identify those with germ cell-specific expression of fluorescent reporter genes that will enable us to isolate spermatogonia using fluorescence-activated cell sorting (FACS). The results in this thesis describe these efforts, and document the identification of an excellent mouse model for future studies in our laboratory to understand the mechanisms underlying spermatogonial development. This work was supported by a grant from the NIH/NICHD (HD090083) to C.B.G.
dc.embargo.lift2020-08-01
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10342/6969
dc.language.isoen
dc.publisherEast Carolina University
dc.subject.meshSpermatogonia
dc.subject.meshAnimals
dc.subject.meshMice, Transgenic
dc.subject.meshMammals
dc.subject.meshSpermatogenesis
dc.titleANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA
dc.typeMaster's Thesis
dc.type.materialtext

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Anatomy and Cell Biology