Genome wide identification of ARF transcription factor gene family and their expression analysis in sweet potato

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dc.access.optionOpen Access
dc.contributor.advisorZhang, Baohong
dc.contributor.authorPratt, Isaac
dc.contributor.departmentBiology
dc.date.accessioned2021-09-02T13:21:09Z
dc.date.available2022-07-01T08:01:55Z
dc.date.created2021-07
dc.date.issued2021-07-15
dc.date.submittedJuly 2021
dc.date.updated2021-08-30T15:41:33Z
dc.degree.departmentBiology
dc.degree.disciplineMS-Biology
dc.degree.grantorEast Carolina University
dc.degree.levelMasters
dc.degree.nameM.S.
dc.description.abstractAuxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 26 possible ItfARF genes were identified in Ipomea trifida; however, ItfARF1a and ItfARF2a were found to be a single gene, resulting in a total of 25 ItfARF genes. Firstly, a comprehensive bioinformatics analysis of the ItfARF gene family was given, including conserved motifs, chromosomal locations, phylogenetic relationships, and a protein data breakdown. Furthermore, the expression patterns of ItfARF genes were analyzed within the storage roots and normal roots, at an early stage of development. ItfARF16b [and] ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root, but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes.
dc.embargo.lift2022-07-01
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10342/9371
dc.language.isoen
dc.publisherEast Carolina University
dc.subjectauxin response factor
dc.subject.lcshTranscription factors
dc.subject.lcshSweet potatoes--Genetics
dc.subject.lcshRoots (Botany)--Development
dc.titleGenome wide identification of ARF transcription factor gene family and their expression analysis in sweet potato
dc.typeMaster's Thesis
dc.type.materialtext

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