Browsing Microbiology and Immunology by Author "Pesci, Everett C."
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Autolysis and Autoaggregation in Pseudomonas aeruginosa Colony Morphology Mutants
D'Argenio, David A.; Calfee, M. Worth; Rainey, Paul B.; Pesci, Everett C. (East Carolina University, 2002-12)Two distinctive colony morphologies were noted in a collection of Pseudomonas aeruginosa transposon insertion mutants. One set of mutants formed wrinkled colonies of autoaggregating cells. Suppressor analysis of a subset ... -
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa
Gallagher, Larry A.; McKnight, Susan L.; Kuznetsova, Marina S.; Pesci, Everett C.; Manoil, Colin (East Carolina University, 2002-12)A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in ... -
Genome Sequences for Two Acinetobacter baumannii Strains Obtained Using the Unicycler Hybrid Assembly Pipeline
Farrow, John M. ,III; Pesci, Everett C.; Slade, Daniel (2021-03-11) -
The Influence of Iron on Pseudomonas aeruginosa Physiology: A regulatory link between iron and quorum sensing
Oglesby, Amanda G.; Farrow, John M. III; Lee, Joon-Hee; Tomaras, Andrew P.; Greenberg, E. Peter; Pesci, Everett C.; Vasil, Michael L. (East Carolina University, 2008-06-06)In iron-replete environments, the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein represses expression of two small regulatory RNAs encoded by prrF1 and prrF2. Here we describe the effects of iron and PrrF ... -
Inhibition of Quorum Sensing by a Pseudomonas aeruginosa dksA Homologue
Branny, Pavel; Pearson, James P.; Pesci, Everett C.; Kohler, Thilo; Iglewski, Barbara H.; Van Delden, Christian (East Carolina University, 2001-03)The Pseudomonas aeruginosa las (lasR-lasI) and rhl (rhlR-rhlI) quorum-sensing systems regulate the expression of several virulence factors, including elastase and rhamnolipid. P. aeruginosa strain PR1-E4 is a lasR deletion ... -
Interference with Pseudomonas quinolone signal synthesis inhibits virulence factor expression by Pseudomonas aeruginosa
Calfee, M. Worth; Coleman, James P.; Pesci, Everett C. (East Carolina University, 2001-09-25)Pseudomonas aeruginosa is an opportunistic pathogen that controls numerous virulence factors through intercellular signals. This bacterium has two quorum-sensing systems (las and rhl), which act through the intercellular ... -
Layered regulation of a pathway capable of altering quinolone production in Pseudomonas aeruginosa
Tipton, Kyle A. (East Carolina University, 2014)Pseudomonas aeruginosa is a gram negative opportunistic pathogen which commonly infects hospitalized patients and those afflicted with cystic fibrosis. P. aeruginosa communicates intercellularly via a system of small ... -
PqsE Functions Independently of PqsR-Pseudomonas Quinolone Signal and Enhances the rhl Quorum-Sensing System
Farrow, John M. III; Sund, Zoe M.; Ellison, Matthew L.; Wade, Dana S.; Coleman, James P.; Pesci, Everett C. (East Carolina University, 2008-11)Pseudomonas aeruginosa is an opportunistic pathogen that causes both acute and chronic infections in immunocompromised individuals. This gram-negative bacterium produces a battery of virulence factors that allow it to ... -
Pseudomonas aeruginosa PqsA Is an Anthranilate-Coenzyme A Ligase
Coleman, James P.; Hudson, L. Lynn; McKnight, Susan L.; Farrow, John M. III; Calfee, M. Worth; Lindsey, Claire A.; Pesci, Everett C. (East Carolina University, 2008-02)Pseudomonas aeruginosa is an opportunistic human pathogen which relies on several intercellular signaling systems for optimum population density-dependent regulation of virulence genes. The Pseudomonas quinolone signal ... -
Pseudomonas aeruginosa relA Contributes to Virulence in Drosophila melanogaster
Erickson, David L.; Lines, J. Louise; Pesci, Everett C.; Venturi, Vittorio; Storey, Douglas G. (East Carolina University, 2004-10)The stringent response is a mechanism by which bacteria adapt to nutritional deficiencies through the production of the guanine nucleotides ppGpp and pppGpp, produced by the RelA enzyme. We investigated the role of the ... -
The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa
McKnight, Susan L.; Iglewski, Barbara H.; Pesci, Everett C. (East Carolina University, 2000-05)The opportunistic pathogen Pseudomonas aeruginosa uses intercellular signals to control the density-dependent expression of many virulence factors. The las and rhl quorum-sensing systems function, respectively, through the ... -
Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa
Pesci, Everett C.; Milbank, Jared B. J.; Pearson, James P.; McKnight, Susan L.; Kende, Andrew S.; Greenberg, E. Peter; Iglewski, Barbara H. (East Carolina University, 1999-09-28)Numerous species of bacteria use an elegant regulatory mechanism known as quorum sensing to control the expression of specific genes in a cell-density dependent manner. In Gram-negative bacteria, quorum sensing ... -
The regulation of anthranilate synthesis for the production of pqs in pseudomonas aeruginosa
Knoten, Claire A. (East Carolina University, 2013)The ubiquitous bacterium P. aeruginosa is an important human pathogen that causes devastating infections in immunocompromised patients and chronic infections in cystic fibrosis patients. The ability of P. aeruginosa to ... -
Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa
Wade, Dana S.; Calfee, M. Worth; Rocha, Edson R.; Ling, Elizabeth A.; Engstrom, Elana; Coleman, James P.; Pesci, Everett C. (East Carolina University, 2005-07)Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in cystic fibrosis patients and is a major source of nosocomial infections. This bacterium controls many virulence factors by using ... -
Solubility and Bioactivity of the Pseudomonas Quinolone Signal Are Increased by a Pseudomonas aeruginosa-Produced Surfactant
Calfee, M. Worth; Shelton, John G.; McCubrey, James A.; Pesci, Everett C. (East Carolina University, 2005-02)Pseudomonas aeruginosa is a gram-negative bacterium that causes serious infections in immunocompromised individuals and cystic fibrosis patients. This opportunistic pathogen controls many of its virulence factors and ... -
Transcriptional regulation of 'pqsR', a gene that encodes a regulator of quinolone signal synthesis and virulence in 'Pseudomonas aeruginosa'
Farrow, John M. III (East Carolina University, 2015)The gram-negative bacterium 'Pseudomonas aeruginosa' is an opportunistic pathogen that frequently causes nosocomial infections and serious chronic lung infections in cystic fibrosis patients. During infection 'P. aeruginosa' ... -
The Transcriptional Regulator Np20 Is the Zinc Uptake Regulator in
Ellison, Matthew L.; Farrow, John M. III; Parrish, Whitney; Danell, Allison Stokes; Pesci, Everett C. (2013)Zinc is essential for all bacteria, but excess amounts of the metal can have toxic effects. To address this, bacteria have developed tightly regulated zinc uptake systems, such as the ZnuABC zinc transporter which is ...