Browsing Biochemistry and Molecular Biology by Author "Chalovich, Joseph"
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The Actin Binding Protein, Fesselin, is a Member of the Synaptopodin Family
Schroeter, Mechthild M.; Beall, Brent; Heid, Hans W.; Chalovich, Joseph (East Carolina University, 2008-07)Fesselin is a natively unfolded protein that is abundant in avian smooth muscle. Like many natively unfolded proteins, fesselin has multiple binding partners including actin, myosin, calmodulin and α-actinin. Fesselin ... -
Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications
Gafurov, Boris; Chen, Yi-Der; Chalovich, Joseph (East Carolina University, 2004-09)Skeletal and cardiac muscle contraction are inhibited by the actin-associated complex of tropomyosin-troponin. Binding of Ca21 to troponin or binding of ATP-free myosin to actin reverses this inhibition. Ca21 and ATP-free ... -
Caldesmon Ablation in Mice Causes Umbilical Herniation and Alters Contractility of Fetal Urinary Bladder Smooth Muscle
Putz, Sandra; Barthel, Lisa Sophie; Frohn, Marina; Metzler, Doris; Barham, Mohammed; Pryymachuk, Galyna; Trunschke, Oliver; Lubomirov, Lubomir T.; Hescheler, Jürgen; Chalovich, Joseph; Neiss, Wolfram F.; Koch, Manuel; Schroeter, Mechthild M.; Pfitzer, Gabriele (2021-04-21) -
Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.
Leinweber, Barbara; Tang, Jay X.; Stafford, Walter F.; Chalovich, Joseph (East Carolina University, 1999-12)The purpose of this study was to address the paradox of calponin localization with a-actinin and filamin, two proteins with tandem calponin homology (CH) domains, by determining the effect of these proteins on the binding ... -
Characterization of a caldesmon fragment that competes with myosin-ATP binding to actin.
Velaz, Laly; Chen, Yi-Der; Chalovich, Joseph (East Carolina University, 1993-08)The protein caldesmon inhibits actin-activated ATP hydrolysis of myosin and inhibits the binding of myosin*ATP to actin. Afragment isolated from a chymotryptic digest of caldesmon contains features of the intact molecule ... -
Conservation of the regulated structure of folded myosin 2 in species separated by at least 600 million years of independent evolution
Jung, Hyun Suk; Burgess, Stan A.; Billington, Neil; Colegrave, Melanie; Patel, Hitesh; Chalovich, Joseph; Chantler, Peter D.; Knight, Peter J. (East Carolina University, 2008-04-22)The myosin 2 family of molecular motors includes isoforms regulated in different ways. Vertebrate smooth-muscle myosin is activated by phosphorylation of the regulatory light chain, whereas scallop striated adductor-muscle ... -
The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin
Gafurov, Boris; Fredricksen, Scott; Cai, Anmei; Brenner, Bernhard; Chase, P. Bryant; Chalovich, Joseph (East Carolina University, 2004-12-07)The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca2+ concentrations. Ca2+ stimulates ATP activity, and at ... -
Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity
Cory, Ann H.; Shibley, Ivan A.; Chalovich, Joseph; Cory, Joseph G. (East Carolina University, 1993-01-05)A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. The deoxyguanosine-resistant cells (dGuo-R) were 126-fold less sensitive to deoxyguanosine than the wild-type cells. The IC50 values for araC ... -
The Distribution Of Regulated Actomyosin States Is Central To Cardiac Muscle Regulation And Disturbance Of This Distribution Leads To Congenital Cardiomyopathies
Mathur, Mohit C. (East Carolina University, 2009)Hypertrophic and restrictive cardiomyopathies are congenital cardiac diseases that have an incidence of over one in five hundred and may lead to sudden cardiac death. One of the main impediments to directed treatment is ... -
Equilibration and Exchange of Fluorescently Labeled Molecules in Skeletal Muscle Fibers Studied Using Confocal Microscopy
Kraft, T.; Messerli, M.; Rutishauser, B.; Wallimann, T.; Perriard, J.-C.; Chalovich, Joseph; Brenner, Bernhard (East Carolina University, 1995-04)Diffusion of molecules into skinned muscle fibers is often necessary when studying muscle contraction and its regulation. Usually, it is assumed that diffusion of molecules is fast also in the structured system of a muscle ... -
Fesselin and caldesmon: natively unfolded proteins in smooth muscle regulation
Hamden, Svetlana (East Carolina University, 2010)Dysregulation of smooth muscle contraction is linked to diseases such as atherosclerosis, asthma, hypertension, urinary incontinence, premature birth and others. The goal of this project is to contribute to our understanding ... -
Fesselin, a Synaptopodin-like Protein, Stimulates Actin Nucleation and Polymerization
Beall, Brent; Chalovich, Joseph (East Carolina University, 2001-11-27)Fesselin is a proline-rich actin binding protein that has recently been isolated from smooth muscle [Leinweber, B. D., Fredricksen, R. S., Hoffman, D. R., and Chalovich, J. M. (1999) J. Muscle Res. Cell Motil. 20, 539–545]. ... -
Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1
Wirth, A.; Schroeter, M.; Kock-Hauser, C.; Manser, E.; Chalovich, Joseph; de Lanerolle, P.; Pfitzer, G. (East Carolina University, 2003-06-01)The p21-activated protein kinases (PAKs) have been implicated in cytoskeletal rearrangements and modulation of non-muscle contractility. Little, however, is known about the role of the PAK family members in smooth muscle ... -
The Interaction of Caldesmon with the COOH Terminus of Actin
Crosbie, Rachelle; Adams, Susan; Chalovich, Joseph; Reisler, Emil (East Carolina University, 1991-10-25)Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon ... -
Kinetics of Binding of Caldesmon to Actin
Chalovich, Joseph; Chen, Yi-Der; Dudek, Ronald W.; Hai, Luo (East Carolina University, 1995-04-28)The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ... -
Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity
Chalovich, Joseph; Bryan, Joseph; Benson, Caryl E.; Velaz, Laly (East Carolina University, 1992-08-15)Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several ... -
A Long Helix from the Central Region of Smooth Muscle Caldesmon
Wang, C.-L. Albert; Chalovich, Joseph; Graceffa, Philip; Lu, Renne C.; Mabuchi, Katsuhide; Stafford, Walter F. (East Carolina University, 1991-07-25)The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic properties and the electron ... -
Negative Charges at Protein Kinase C Sites of Troponin I Stabilize the Inactive State of Actin
Mathur, Mohit C.; Kobayashi, Tomoyoshi; Chalovich, Joseph (East Carolina University, 2008-01-15)Alterations in the troponin complex can lead to increases or decreases in contractile activity. Most mutations of troponin that cause hypertrophic cardiomyopathy increase the activity of cardiac muscle fibers. In at least ... -
Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State
Mathur, Mohit C.; Kobayashi, Tomoyoshi; Chalovich, Joseph (East Carolina University, 2009-03-18)We examined four cardiomyopathy-causing mutations of troponin I that appear to disturb function by altering the distribution of thin filament states. The R193H (mouse) troponin I mutant had greater than normal actin-activated ... -
Structural Dynamics of Troponin I during Ca2+-Activation of Cardiac Thin Filaments: A Multi-Site Förster Resonance Energy Transfer Study
Wang, Hui; Chalovich, Joseph; Marriott, Gerard (2012)