Browsing by Author "Allen, William E."
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A Carbohydrate/Lipid Research Project for Organic and Analytical CURE Laboratories: Reactions and Recordkeeping
Hagwood, Abigail M (East Carolina University, 2021-05-06)Course-based Undergraduate Research Experiences (or CUREs) are able to teach students laboratory skills while engaging them in novel research. The first part of this thesis aims to demonstrate the development of a feasible ... -
AZULENE/ALDEHYDE ASSOCIATION: AN ANALOG FOR DAMAGED DNA DETECTION
Shields, Agne A (East Carolina University, 2022-12-14)Loss of a nucleic acid base from DNA can leave behind a reactive aldehyde group on ribose. To detect the presence of such “abasic sites,” ethyl-2-aminoazulene-1-carboxylate, a novel azulene-based analog of guanine/adenine, ... -
Biimidazole diamide anion binding agents
Allen, William E.; Causey, Corey (2006-07-18)Biimidazole diamide compounds that are useful for binding anions such as sulfate are described, along with the use thereof for purposes such as extracting anions from a waste stream and/or detecting anions. -
Elucidating "Consumption" : Using Fluorescent Steroid Probes to Understand Host Cholesterol Utilization by Mycobacterium spp.
Wercholuk, Ashley N. (East Carolina University, 2014)Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB) disease, is the leading cause of death due to bacterial infection worldwide, claiming nearly two million lives each year. Often, the immune system ... -
Fluorescent Derivatives of Prostamides: Tools for Studying Anti-Cancer Activity
Stanley, Jordan Lynne (East Carolina University, 2018-07-23)The endocannabinoid arachidonoyl ethanolamide (AEA) and prostaglandins derived from it selectively induce apoptosis in tumorigenic cell lines due to overexpression of COX-2. It is likely that a novel J-series prostamide, ... -
HOW CHARGED RESIDUES INFLUENCE THE THERMAL STABILITY OF COLLAGEN: A STUDY WITH NATURAL AND NON-NATURAL AMINO ACIDS
Banzon, Patrick D. (East Carolina University, 2020-09-16)Triple-helical collagens are key structural proteins in mammals. Their ubiquity and diverse functions drive our interest into understanding their behavior at a fundamental level. This thesis describes a reductionist approach ... -
Installation of Metal-Binding Functionality at Lysine Residues via Azide-Alkyne "Click Chemistry"
Baucom, Matthew (2015-04-29)A novel non-natural amino acid designed to bind divalent metal ions, dubbed Lys(PYRIT), was prepared in a “click reaction” by treating Fmoc-Lys(N3)-OH with 2-ethynylpyridine and Cu(I). It was incorporated into a 12-residue ... -
Investigation of Platinum-Catalyzed Acylation of 2-Aryloxypyridines via C-H Activation and Functionalization
Barham, Alex Meacham (East Carolina University, 2022-07-12)Transition metal catalyzed C-H activation and functionalization reactions allow synthetic chemists to break the normally unreactive carbon-hydrogen bond and create a new and useful carbon-oxygen, carbon-nitrogen, carbon-halogen, ... -
Membrane Localization of Pyrene-Trehalose Conjugates (PYRETs)
Hagwood, Abigail (East Carolina University, 2018-05-01)The disaccharide trehalose is found in many organisms that can survive in extremely cold or dry environments. According to the “hydration hypothesis,” trehalose interacts with the glycerol/phosphate regions of lipid bilayers ... -
Recognition of bacterial lipid headgroups by fluorescent crown ether-naphthalimides
Marshall, Sarah Ruth (2015)The increasing incidence of antibiotic- resistant bacterial strains is a significant threat to human health. New antimicrobial mechanisms that feature reduced resistance potential are necessary to slow down the rapid ... -
Square Wave Voltammetry Detection and CD Spectroscopy Structural Characterization of 5- Methyl Cytosine-Containing Oligomeric DNA Sequences
Preston, Victoria G (East Carolina University, 2023-01-04)DNA methylation, primarily at the 5-carbon in cytosine (5-methyl cytosine) plays a significant role in key processes and functions of the body, such as development of the brain and in neuronal cell differentiation. Lack ... -
STABILIZATION OF THE COLLAGEN TRIPLE HELIX WITH A RUTHENIUM(II) ANCHOR
Banzon, Patrick D. (East Carolina University, 2017-05-04)Two collagen analogues based on a (Pro-Hyp-Gly)7 core were given metal-binding ability by linking histidine to either the N- or C- termini using a six-carbon spacer. Circular dichroism studies confirmed that the isomers, ... -
SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF BOC- PROTECTED FLUORESCENT ARGININE
Marshall, Sarah Ruth (East Carolina University, 2017-08-21)Small peptides capable of penetrating or spanning the hydrophobic core of lipid bilayers have many potential uses in medicine and in biochemical research. As the most basic of the amino acids that can comprise such peptides, ... -
Synthesis Of Non-Natural Fmoc-Protected Amino Acids To Provide Novel Fluorescent Anion Probes And Their Incorporation Into Synthetic Peptides
Farrell, David Percy (East Carolina University, 2015)Cystic fibrosis is a genetic disorder caused by mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. CFTR serves to control the gradient of chloride and bicarbonate ions across the cellular ... -
Synthetic Antimicrobial Peptides Exhibit Two Different Binding Mechanisms to the Lipopolysaccharides Isolated from and
Chai, Hanbo; Allen, William E.; Hicks, Rickey P. (2014) -
WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD
McGrath, Jacqueline (East Carolina University, 2010)Intracellular pH plays an important role in many biological processes: receptor-mediated signal transduction, enzymatic activity, cell growth and death, ion transport and homeostasis, calcium regulation, endocytosis, ...