Browsing by Author "Brickman, Timothy J."
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Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants.
Brickman, Timothy J.; Armstrong, Sandra K. (East Carolina University, 1995-01)We report the isolation and preliminary phenotypic characterization of manganese-resistant Bordetella bronchiseptica mutants with respect to deregulation of siderophore and iron-regulated protein expression. The fur gene ... -
Essential Role of the Iron-Regulated Outer Membrane Receptor FauA in Alcaligin Siderophore-Mediated Iron Uptake in Bordetella Species
Brickman, Timothy J.; Armstrong, Sandra K. (East Carolina University, 1999-10)Phenotypic analysis using heterologous host systems localized putative Bordetella pertussis ferric alcaligin transport genes and Fur-binding sequences to a 3.8-kb genetic region downstream from the alcR regulator gene. ... -
Identification and Characterization of alcR, a Gene Encoding an AraC-Like Regulator of Alcaligin Siderophore Biosynthesis and Transport in Bordetella pertussis and Bordetella bronchiseptica
Beaumont, Fiona C.; Kang, Ho Young; Brickman, Timothy J.; Armstrong, Sandra K. (East Carolina University, 1998-02)A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin ... -
Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.
Kang, Ho Young; Brickman, Timothy J.; Beaumont, Fiona C.; Armstrong, Sandra K. (East Carolina University, 1996-08)Bordetella bronchiseptica mutants BRM1, BRM6, and BRM9 fail to produce the native dihydroxamate siderophore alcaligin. A 4.5-kb BamHI-SmaI Bordetella pertussis genomic DNA fragment carried multiple genes required to restore ... -
The ornithine decarboxylase gene odc is required for alcaligin siderophore biosynthesis in Bordetella spp.: putrescine is a precursor of alcaligin.
Brickman, Timothy J.; Armstrong, Sandra K. (East Carolina University, 1996-01)Chromosomal insertions defining Bordetella bronchiseptica siderophore phenotypic complementation group III mutants BRM3 and BRM5 were found to reside approximately 200 to 300 bp apart by restriction mapping of cloned genomic ... -
Transcriptional Activation of Bordetella Alcaligin Siderophore Genes Requires the AlcR Regulator with Alcaligin as Inducer
Brickman, Timothy J.; Kang, Ho Young; Armstrong, Sandra K. (East Carolina University, 2001-01)Genetic and biochemical studies have established that Fur and iron mediate repression of Bordetella alcaligin siderophore system (alc) genes under iron-replete nutritional growth conditions. In this study, transcriptional ...