Browsing by Author "Setlow, Peter"
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Analysis of the Germination of Individual Clostridium sporogenes Spores with and without Germinant Receptors and Cortex-Lytic Enzymes
Wang, Shiwei; Brunt, Jason; Peck, Michael W.; Setlow, Peter; Li, Yong-Qing (2017-10-25)The Gram-positive spore-forming anaerobe Clostridium sporogenes is a significant cause of food spoilage, and it is also used as a surrogate for C. botulinum spores for testing the efficacy of commercial sterilization. C. ... -
Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus 2 Subtilis Cells Upon Prophage Induction During Spore Germination and 3 Outgrowth, and Vegetative Growth, Using Raman Tweezers and Live-Cell 4 Imaging
Wu, Mei-yan; Li, Yong-qing; Li, Wen-wei; Christie, Graham; Setlow, Peter (2021) -
Characterization of Wet-Heat Inactivation of Single Spores of Bacillus Species by Dual-Trap Raman Spectroscopy and Elastic Light Scattering
Zhang, Pengfei; Kong, Lingbo; Setlow, Peter; Li, Yong-qing (2010-03)Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to investigate dynamic processes during high-temperature treatment of individual spores of Bacillus cereus, Bacillus megaterium, ... -
A Clostridium difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination
Donnelly, M. Lauren; Li, William; Li, Yong-qing; Hinkel, Lauren; Setlow, Peter; Shen, Aimee (2017-01-17)Clostridium difficile is a Gram-positive spore-forming obligate anaerobe that is a leading cause of antibiotic-associated diarrhea worldwide. In order for C. difficile to initiate infection, its aerotolerant spore form ... -
Combination of Raman tweezers and quantitative differential interference contrast microscopy for measurement of dynamics and heterogeneity during the germination of individual bacterial spores
Zhang, Pengfei; Kong, Lingbo; Wang, Guiwen; Setlow, Peter; Li, Yong-Qing (2010-09-01)Raman tweezers and quantitative differential interference contrast (DIC) microscopy are combined to monitor the dynamic germination of individual bacterial spores of Bacillus species, as well as the heterogeneity in this ... -
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics
Peng, Lixin; Chen, De; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2009-05-15)Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneously measure levels of Ca-dipicolinic acid (CaDPA) and changes in spore morphology and refractive index during germination ... -
How Moist Heat Kills Spores of Bacillus subtilis
Coleman, William H.; Chen, De; Li, Yong-Qing; Cowan, Ann E.; Setlow, Peter (East Carolina University, 2007-12)Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores ... -
Kinetics of Germination of Individual Spores of as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Zhou, Tingting; Dong, Zhiyang; Setlow, Peter; Li, Yong-Qing (2013)Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of ... -
Kinetics of Germination of Individual Spores of Geobacillus stearothermophilus as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Zhou, Tingting; Dong, Zhiyang; Setlow, Peter; Li, Yong-qing (2013-09-13)Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of ... -
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2007-07)Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many ... -
Memory of Germinant Stimuli in Bacterial Spores
Wang, Shiwei; Faeder, James R.; Setlow, Peter; Li, Yong-Qing (2015-11)Bacterial spores, despite being metabolically dormant, possess the remarkable capacity to detect nutrients and other molecules in their environment through a biochemical sensory apparatus that can trigger spore germination, ... -
Memory of Germinant Stimuli in Bacterial Spores
Wang, Shiwei; Faeder, James R.; Setlow, Peter; Li, Yong-qing (2015-11)Bacterial spores, despite being metabolically dormant, possess the remarkable capacity to detect nutrients and other molecules in their environment through a biochemical sensory apparatus that can trigger spore germination, ... -
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
Magge, Anil; Granger, Amanda C.; Wahome, Paul G.; Setlow, Barbara; Vepachedu, Venkata R.; Loshon, Charles A.; Peng, Lixin; Chen, De; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2008-07)Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these ... -
Single-cell analysis reveals individual spore responses to simulated space vacuum
He, Lin; Wang, Shiwei; Cortesão, Marta; Wu, Muying; Moeller, Ralf; Setlow, Peter; Li, Yong-qing (2018-12-04)Outer space is a challenging environment for all forms of life, and dormant spores of bacteria have been frequently used to study the survival of terrestrial life in a space journey. Previous work showed that outer space ... -
Superdormant Spores of Bacillus Species Have Elevated Wet-Heat Resistance and Temperature Requirements for Heat Activation
Ghosh, Sonali; Zhang, Pengfei; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2009-09)Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, D-glucose, or L-valine, respectively, germinate ... -
Uptake of and Resistance to the Antibiotic Berberine by Individual Dormant, Germinating and Outgrowing Spores as Monitored by Laser Tweezers Raman Spectroscopy
Wang, Shiwei; Yu, Jing; Suvira, Milomir; Setlow, Peter; Li, Yong-Qing (2015-12)Article Authors Metrics Comments Related Content Abstract Introduction Materials and Methods Results Discussion Supporting Information Acknowledgments Author Contributions References Reader Comments (0) Media ... -
Uptake of and resistance to the antibiotic berberine by individual dormant, germinating and outgrowing bacillus spores as monitored by laser tweezers raman spectroscopy
Wang, Shiwei; Yu, Jing; Suvira, Milomir; Setlow, Peter; Li, Yong-qing (2015-12-04)Berberine, an alkaloid originally extracted from the plant Coptis chinensis and other herb plants, has been used as a pharmacological substance for many years. The therapeutic effect of berberine has been attributed to ...